| Circulating bile salt-dependent lipase originates from the pancreas via intestinal transcytosis. | |
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MedLine Citation:
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PMID: 12557152 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND & AIMS: Bile salt-dependent lipase (BSDL) has been detected in human blood, where it is assumed to play a substantial role in atherosclerosis. The origin of this circulating enzyme is unknown. The aim of this study was to show that blood BSDL originates from pancreatic exocrine secretions via a transcytotic motion across the intestinal epithelium. METHODS: Fluorescein isothiocyanate- or [(125)I]-labeled human pancreatic BSDL was instilled into the lumen of intestinal loops of the rat, and combined biochemical and immunocytochemical investigations were performed in intestinal cells and in the blood of these animals. RESULTS: In vivo pancreatic BSDL is internalized by duodenal enterocytes. The pancreatic enzyme was associated with microvilli and present in endocytic vesicles and Golgi apparatus as well as along the basolateral membrane of enterocytes. It was also detected in intestinal interstitial spaces. Radiolabeled pancreatic BSDL internalized by the duodenal epithelium is the one further detected in circulation. The radiolabeled protein was immunoprecipitated from plasma and had a 100-kilodalton molecular mass compatible with native pancreatic BSDL. In blood, BSDL was mainly associated with low-density lipoproteins. CONCLUSIONS: These in vivo data show that BSDL, normally present in blood, originates from exocrine pancreatic secretion and support the pathophysiologic relevance of BSDL transcytosis through the intestinal mucosa cell lining. Based on this, the implication of circulating BSDL in atherosclerosis merits careful attention. |
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Authors:
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Nadine Bruneau; Moïse Bendayan; Diane Gingras; Lucien Ghitescu; Emile Levy; Dominique Lombardo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gastroenterology Volume: 124 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-01-30 Completed Date: 2003-03-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: United States |
Other Details:
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Languages: eng Pagination: 470-80 Citation Subset: AIM; IM |
Affiliation:
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INSERM U559, Unité de Recherche de Physiopathologie des Cellules Epithéliales and EA 3289, Université de la Mediterranée, Faculté de Médecine-Timone, Marseilles, France. Nadine.Bruneau@medecine.univ-mrs.fr |
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Cattle Enterocytes / metabolism* Fluorescein-5-isothiocyanate Fluorescent Dyes Humans Intestinal Mucosa / metabolism* Lipoproteins / metabolism Male Pancreas / metabolism* Pancreatic Juice / metabolism Rats Rats, Sprague-Dawley Sterol Esterase / blood, metabolism*, pharmacokinetics Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Lipoproteins; 3326-32-7/Fluorescein-5-isothiocyanate; EC 3.1.1.-/bile salt-stimulated lipase; EC 3.1.1.13/Sterol Esterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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