Document Detail


Circulating proteasomes after burn injury.
MedLine Citation:
PMID:  20182370     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of the study is to test whether circulating proteasomes are increased in burn patients and to assess whether possible alterations are associated with severity of injury, organ failure, and/or clinically relevant outcomes. In this study, plasma was obtained from burn patients on days 0 (admission, n = 50), 1 (n = 36), 3 (n = 35), 5 (n = 28), 7 (n=34), and 30 (n = 10) (controls: 40 volunteers). The 20S/26S proteasome levels were measured by enzyme-linked immunosorbent assay. Proteasome peptidase activity was assessed using a chymotryptic-like peptide substrate in combination with epoxomicin (specific proteasome inhibitor). Percentage of TBSA burned, presence of inhalation injury, development of sepsis/multiple organ failure, and sequential organ failure assessment scores were documented. On admission, plasma proteasome activity was higher in patients than in controls (P = .011). 26S proteasomes were not detectable. The 20S proteasome concentrations (median [25th/75th percentile]) peaked on day 0 (673 [399/1566] ng/mL; control: 195 [149/249] ng/mL, P < .001), gradually declined within 7 days, and fully returned to baseline at day 30 (116.5 [78/196] ng/mL). Elevated 20S proteasomes were associated with the presence of inhalation injury and correlated linearly with %TBSA in patients without inhalation injury. Initial 20S proteasome concentrations discriminated the presence of inhalation injury in patients with (sensitivity 0.88 and specificity 0.71) and without (sensitivity 0.83 and specificity 0.97) cutaneous burns but did not discriminate sepsis/multiple organ failure development or survival. Circulating 20S proteasome is a biomarker of tissue damage. The 20S proteasome plasma concentrations in patients with burns and/or inhalation injury are unlikely to predict outcomes but may be useful for the diagnosis of inhalation injury.
Authors:
Matthias Majetschak; Siegfried Zedler; Jacqueline Romero; Joslyn M Albright; Robert Kraft; Elizabeth J Kovacs; Eugen Faist; Richard L Gamelli
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of burn care & research : official publication of the American Burn Association     Volume:  31     ISSN:  1559-0488     ISO Abbreviation:  J Burn Care Res     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-03-02     Completed Date:  2010-06-17     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  101262774     Medline TA:  J Burn Care Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-50     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Burns / enzymology*
Case-Control Studies
Enzyme-Linked Immunosorbent Assay
Female
Humans
Injury Severity Score
Male
Multiple Organ Failure / enzymology
Oligopeptides
Proteasome Endopeptidase Complex / blood*
ROC Curve
Regression Analysis
Sensitivity and Specificity
Sepsis / enzymology
Smoke Inhalation Injury / enzymology
Statistics, Nonparametric
Grant Support
ID/Acronym/Agency:
AG018859/AG/NIA NIH HHS; R01 AA012034/AA/NIAAA NIH HHS; R01 AA012034/AA/NIAAA NIH HHS; R01 AA012034-10/AA/NIAAA NIH HHS; R01 AA012034-11/AA/NIAAA NIH HHS; R01 AG018859/AG/NIA NIH HHS; R01 AG018859-10/AG/NIA NIH HHS; T32 AA013527/AA/NIAAA NIH HHS; T32 AA013527-08/AA/NIAAA NIH HHS; T32 AA013527-09/AA/NIAAA NIH HHS; T32 GM008750/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Oligopeptides; 134381-21-8/epoxomicin; EC 3.4.25.1/Proteasome Endopeptidase Complex
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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