| Circulating microRNAs in patients with coronary artery disease. | |
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MedLine Citation:
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PMID: 20595655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RATIONALE: MicroRNAs are small RNAs that control gene expression. Besides their cell intrinsic function, recent studies reported that microRNAs are released by cultured cells and can be detected in the blood. OBJECTIVE: To address the regulation of circulating microRNAs in patients with stable coronary artery disease. METHODS AND RESULTS: To determine the regulation of microRNAs, we performed a microRNA profile using RNA isolated from n=8 healthy volunteers and n=8 patients with stable coronary artery disease that received state-of-the-art pharmacological treatment. Interestingly, most of the highly expressed microRNAs that were lower in the blood of patients with coronary artery disease are known to be expressed in endothelial cells (eg, miR-126 and members of the miR-17 approximately 92 cluster). To prospectively confirm these data, we detected selected microRNAs in plasma of 36 patients with coronary artery disease and 17 healthy volunteers by quantitative PCR. Consistent with the data obtained by the profile, circulating levels of miR-126, miR-17, miR-92a, and the inflammation-associated miR-155 were significantly reduced in patients with coronary artery disease compared with healthy controls. Likewise, the smooth muscle-enriched miR-145 was significantly reduced. In contrast, cardiac muscle-enriched microRNAs (miR-133a, miR-208a) tend to be higher in patients with coronary artery disease. These results were validated in a second cohort of 31 patients with documented coronary artery disease and 14 controls. CONCLUSIONS: Circulating levels of vascular and inflammation-associated microRNAs are significantly downregulated in patients with coronary artery disease. |
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Authors:
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Stephan Fichtlscherer; Salvatore De Rosa; Henrik Fox; Thomas Schwietz; Ariane Fischer; Christoph Liebetrau; Michael Weber; Christian W Hamm; Tino Röxe; Marga Müller-Ardogan; Angelika Bonauer; Andreas M Zeiher; Stefanie Dimmeler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-01 |
Journal Detail:
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Title: Circulation research Volume: 107 ISSN: 1524-4571 ISO Abbreviation: Circ. Res. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-03 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: United States |
Other Details:
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Languages: eng Pagination: 677-84 Citation Subset: IM |
Affiliation:
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Institute for Cardiovascular Regeneration, Goethe University Frankfurt, Theodor Stern Kai 7, Frankfurt, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Case-Control Studies Coronary Artery Disease / drug therapy, genetics*, radiography Down-Regulation Female Gene Expression Profiling / methods Genetic Markers* Germany Humans Male MicroRNAs / blood* Middle Aged Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Prospective Studies Reproducibility of Results Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Genetic Markers; 0/MIRN126 microRNA, human; 0/MIRN133 microRNA, human; 0/MIRN145 microRNA, human; 0/MIRN155 microRNA, human; 0/MIRN17 microRNA, human; 0/MIRN208 microRNA, human; 0/MIRN92 microRNA, human; 0/MicroRNAs |
| Comments/Corrections | |
Comment In:
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Circ Res. 2010 Sep 3;107(5):573-4
[PMID:
20814026
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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