Document Detail

Circulating microRNA miR-323-3p as a biomarker of ectopic pregnancy.
MedLine Citation:
PMID:  22395025     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The use of serum human chorionic gonadotropin (hCG) and progesterone to identify patients with ectopic pregnancy (EP) has been shown to have poor clinical utility. Pregnancy-associated circulating microRNAs (miRNAs) have been proposed as potential biomarkers for the diagnosis of pregnancy-associated complications. This proof-of-concept study examined the diagnostic accuracy of various miRNAs to detect EP in an emergency department (ED) setting.
METHODS: This study was a retrospective case-control analysis of 89 women who presented to the ED with vaginal bleeding and/or abdominal pain/cramping and received a diagnosis of viable intrauterine pregnancy (VIP), spontaneous abortion (SA), or EP. Serum hCG and progesterone concentrations were measured by immunoassays. The serum concentrations of miRNAs miR-323-3p, miR-517a, miR-519d, and miR-525-3p were measured with TaqMan real-time PCR. Statistical analysis was performed to determine the clinical utility of these biomarkers, both as single markers and as multimarker panels for EP.
RESULTS: Concentrations of serum hCG, progesterone, miR-517a, miR-519d, and miR-525-3p were significantly lower in EP and SA cases than in VIP cases (P < 0.01). In contrast, the concentration of miR-323-3p was significantly increased in EP cases, compared with SA and VIP cases (P < 0.01). As a single marker, miR-323-3p had the highest sensitivity of 37.0% (at a fixed specificity of 90%). In comparison, the combined panel of hCG, progesterone, and miR-323-3p yielded the highest sensitivity (77.8%, at a fixed specificity of 90%). A stepwise analysis that used hCG first, added progesterone, and then added miR-323-3p yielded a 96.3% sensitivity and a 72.6% specificity.
CONCLUSIONS: Pregnancy-associated miRNAs, especially miR-323-3p, added substantial diagnostic accuracy to a panel including hCG and progesterone for the diagnosis of EP.
Zhen Zhao; Qiuhong Zhao; Joshua Warrick; Christina M Lockwood; Alison Woodworth; Kelle H Moley; Ann M Gronowski
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Publication Detail:
Type:  Journal Article; Multicenter Study; Research Support, N.I.H., Extramural     Date:  2012-03-06
Journal Detail:
Title:  Clinical chemistry     Volume:  58     ISSN:  1530-8561     ISO Abbreviation:  Clin. Chem.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-30     Completed Date:  2012-07-05     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  896-905     Citation Subset:  IM    
Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.
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MeSH Terms
Biological Markers / blood
Case-Control Studies
Chorionic Gonadotropin / blood
MicroRNAs / blood*
Pregnancy, Ectopic / diagnosis*
Progesterone / blood
Real-Time Polymerase Chain Reaction
Retrospective Studies
Sensitivity and Specificity
Grant Support
Reg. No./Substance:
0/Biological Markers; 0/Chorionic Gonadotropin; 0/MIRN323 microRNA, human; 0/MicroRNAs; 57-83-0/Progesterone

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