Document Detail


Circulating microRNA is a biomarker of pediatric Crohn disease.
MedLine Citation:
PMID:  21546856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The gold standard for the diagnosis and evaluation of Crohn disease (CD) is endoscopy/colonoscopy, although this is invasive, costly, and associated with risks to the patient. Recently, circulating microRNAs (miRNAs) have emerged as promising noninvasive biomarkers. Here, we examined the utility of serum miRNAs as biomarkers of CD in children.
PATIENTS AND METHODS: Studies were conducted using sera samples from patients with pediatric CD, healthy controls, and a comparison group of patients with pediatric celiac disease. Serum miRNA levels were explored initially using a microfluidic quantitative reverse transcription-polymerase chain reaction array platform. Findings were subsequently validated using quantitative reverse transcription-polymerase chain reaction in larger validation sample sets. The diagnostic utility of CD-associated serum miRNA was examined using receiver operating characteristic analysis.
RESULTS: A survey of miRNA levels in the sera of control and patients with CD detected significant elevation of 24 miRNAs, 11 of which were chosen for further validation. All of the candidate biomarker miRNAs were confirmed in an independent CD sample set (n = 46). To explore the specificity of the CD-associated miRNAs, they were measured in the sera of patients with celiac disease (n = 12); none were changed compared with healthy controls. Receiver operating characteristic analyses revealed that serum miRNAs have promising diagnostic utility, with sensitivities for CD above 80%. Significant decreases in serum miRNAs were observed in 24 incident patients with pediatric CD after 6 months of treatment.
CONCLUSIONS: The present study identifies 11 CD-associated serum miRNA with encouraging diagnostic potential. Our findings suggest serum miRNAs may prove useful as noninvasive biomarkers in CD.
Authors:
Adam M Zahm; Meena Thayu; Nicholas J Hand; Amber Horner; Mary B Leonard; Joshua R Friedman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  53     ISSN:  1536-4801     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-06-22     Completed Date:  2011-10-31     Revised Date:  2013-12-12    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  26-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Anti-Inflammatory Agents, Non-Steroidal / therapeutic use
Biological Markers / blood
Child
Crohn Disease / blood*,  diagnosis*,  drug therapy
Down-Regulation / drug effects
Drug Monitoring
Female
Humans
Immunosuppressive Agents / therapeutic use
Male
MicroRNAs / blood*
Microfluidic Analytical Techniques
Oligonucleotide Array Sequence Analysis
Pilot Projects
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Severity of Illness Index
Up-Regulation*
Grant Support
ID/Acronym/Agency:
F32 DK093252/DK/NIDDK NIH HHS; K23DK082012/DK/NIDDK NIH HHS; K24 DK076808/DK/NIDDK NIH HHS; R01DK079881/DK/NIDDK NIH HHS; R01KD60030//PHS HHS
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Biological Markers; 0/Immunosuppressive Agents; 0/MicroRNAs
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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