Document Detail

Circulating fibrocytes are increased in children and young adults with pulmonary hypertension.
MedLine Citation:
PMID:  21700605     Owner:  NLM     Status:  MEDLINE    
Chronic inflammation is an important component of the fibroproliferative changes that characterise pulmonary hypertensive vasculopathy. Fibrocytes contribute to tissue remodelling in settings of chronic inflammation, including animal models of pulmonary hypertension (PH). We sought to determine whether circulating fibrocytes were increased in children and young adults with PH. 26 individuals with PH and 10 with normal cardiac anatomy were studied. Fresh blood was analysed by flow cytometry for fibrocytes expressing CD45 and procollagen. Fibrocyte numbers were correlated to clinical and haemodynamic parameters, and circulating CC chemokine ligand (CCL)2 and CXC chemokine ligand (CXCL)12 levels. We found an enrichment of circulating fibrocytes among those with PH. No differences in fibrocytes were observed among those with idiopathic versus secondary PH. Higher fibrocytes correlated to increasing mean pulmonary artery pressure and age, but not to length or type of treatment. Immunofluorescence analysis confirmed flow sorting specificity. Differences in plasma levels of CCL2 or CXCL12, which could mobilise fibrocytes from the bone marrow, were not found. We conclude that circulating fibrocytes are significantly increased in individuals with PH compared with controls. We speculate that these cells might play important roles in vascular remodelling in children and young adults with pulmonary hypertension.
M E Yeager; C M Nguyen; D D Belchenko; K L Colvin; S Takatsuki; D D Ivy; K R Stenmark
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-06-23
Journal Detail:
Title:  The European respiratory journal     Volume:  39     ISSN:  1399-3003     ISO Abbreviation:  Eur. Respir. J.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-02     Completed Date:  2012-06-19     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  8803460     Medline TA:  Eur Respir J     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  104-11     Citation Subset:  IM    
Dept of Paediatrics, Division of Pulmonary and Critical Care Medicine, University of Colorado Denver, Aurora, CO 80045, USA.
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MeSH Terms
Antigens, CD45 / biosynthesis
Case-Control Studies
Cell Separation
Chemokine CCL2 / blood
Chemokine CXCL12 / blood
Fibroblasts / cytology*
Flow Cytometry / methods
Hypertension, Pulmonary / blood*
Mesoderm / cytology*
Phagocytes / cytology*
Stem Cells / cytology
Young Adult
Grant Support
HL-014985-35/HL/NHLBI NIH HHS; HL-084923-02/HL/NHLBI NIH HHS; P50 HL084923-05S1/HL/NHLBI NIH HHS; UL1 RR025780/RR/NCRR NIH HHS
Reg. No./Substance:
0/CXCL12 protein, human; 0/Chemokine CCL2; 0/Chemokine CXCL12; EC, CD45
Comment In:
Eur Respir J. 2012 Jan;39(1):5-6   [PMID:  22210809 ]

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