Document Detail

Circulating benign nevus cells detected by ISET technique: warning for melanoma molecular diagnosis.
MedLine Citation:
PMID:  20956643     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The notion that only malignant melanoma cells circulate and diffuse is shared by oncologists and pathologists. Isolation by size of epithelial tumor cells (ISET) allows the identification of circulating tumor cells by filtration according to size. OBSERVATIONS: During a study of identification of circulating melanoma cells using ISET, blood samples from a 69-year-old man with an atypical melanocytic lesion on his back were evaluated. Binucleated and multinucleated cells that fulfilled the criteria for circulating tumor cells were found. The morphological features were similar to those of the excised skin tissue specimen, and the patient was subsequently diagnosed as having a congenital melanocytic nevus. BRAF (V600E)-mutated DNA was detected in both plasma and formalin-fixed tissue specimens, and the blood samples demonstrated an increase in tyrosinase messenger RNA levels. CONCLUSION: The finding that benign nevus cells may circulate in blood brings into question the value of tyrosinase or other melanocytic markers as a molecular surrogate for circulating melanoma cells.
Vincenzo De Giorgi; Pamela Pinzani; Francesca Salvianti; Marta Grazzini; Claudio Orlando; Torello Lotti; Mario Pazzagli; Daniela Massi
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of dermatology     Volume:  146     ISSN:  1538-3652     ISO Abbreviation:  Arch Dermatol     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-19     Completed Date:  2010-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372433     Medline TA:  Arch Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1120-4     Citation Subset:  AIM; IM    
Department of Dermatology, University of Florence, Via della Pergola 60, 50132 Florence, Italy.
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MeSH Terms
Cell Separation / methods*
Epithelial Cells
Melanoma / secondary*
Molecular Diagnostic Techniques / methods*
Neoplastic Cells, Circulating*
Skin Neoplasms / pathology*
Comment In:
Arch Dermatol. 2010 Oct;146(10):1156-7   [PMID:  20956651 ]

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