| Circular dichroism to determine binding mode and affinity of ligand-DNA interactions. | |
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MedLine Citation:
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PMID: 18079716 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Circular dichroism (CD) is a useful technique for an assessment of DNA-binding mode, being a more accessible, low-resolution complement to NMR and X-ray diffraction methods. Ligand-DNA interactions can be studied by virtue of the interpretation of induced ligand CD signals resulting from the coupling of electric transition moments of the ligand and DNA bases within the asymmetric DNA environment. This protocol outlines methods to determine the binding mode and affinity of ligand-DNA interactions and takes approximately 7.5 h. |
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Authors:
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Nichola C Garbett; Patricia A Ragazzon; Jonathan B Chaires |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature protocols Volume: 2 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2007 |
Date Detail:
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Created Date: 2007-12-14 Completed Date: 2008-02-19 Revised Date: 2008-03-24 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 3166-72 Citation Subset: IM |
Affiliation:
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James Graham Brown Cancer Center, Health Sciences Campus, University of Louisville, 529 S. Jackson Street, Louisville, Kentucky 40202, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Circular Dichroism* DNA / chemistry*, metabolism* Ligands Protein Binding |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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