| Circatidal variation in epithelial cell proliferation in the mussel digestive gland and stomach. | |
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MedLine Citation:
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PMID: 15503161 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epithelial cell renewal in mussel ( Mytilus galloprovincialis, Lmk) digestive gland and stomach was investigated by bromodeoxyuridine (BrdU) immunohistochemistry. Mussels were exposed to 4 mg BrdU/l seawater continuously. Starting at 6 h after treatment, samples were collected every 2 h for 2 days and BrdU labelling was estimated by direct counting at the light microscope, with values being noted per thousand BrdU-positive cells. BrdU-positive reaction was observed in the nuclei of digestive, basophilic, duct and stomach cells, and in haemocytes. Cell renewal in digestive diverticula was synchronised following a circatidal pattern: BrdU labelling increased during low tide and decreased during high tide. Clearcut mitotic figures were identified in digestive cells, thereby confirming that mature cell types proliferate, in agreement with results from immunohistochemistry for proliferating cell nuclear antigen and BrdU. Epithelial cell renewal in the stomach also appeared to be synchronised. |
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Authors:
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B Zaldibar; I Cancio; I Marigómez |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-09-30 |
Journal Detail:
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Title: Cell and tissue research Volume: 318 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-10-25 Completed Date: 2005-03-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 395-402 Citation Subset: IM |
Affiliation:
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Department of Zoology, School of Science and Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Basque Country, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bivalvia / anatomy & histology*, physiology Bromodeoxyuridine / metabolism Cell Count Cell Nucleus / metabolism Cell Proliferation Digestive System / cytology*, metabolism Epithelial Cells / cytology*, metabolism Periodicity* Stomach / cytology, metabolism Time Factors Water Movements* |
| Chemical | |
Reg. No./Substance:
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59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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