| Circadian transcription depends on limiting amounts of the transcription co-activator nejire/CBP. | |
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MedLine Citation:
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PMID: 17635913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The circadian clock orchestrates physiological and behavioral activities, including metabolism, neuronal activity, and cell proliferation in synchrony with the environmental cycle of day and night. Here we show that the Drosophila ortholog of the CBP/p300 family of transcription co-activators, nejire (nej), is an intrinsic component of the circadian clock that performs regulatory functions for circadian controlled transcription. Screening of overexpression mutants revealed that gain of nej function was associated with a loss of behavioral and molecular rhythms. Overexpression of NEJ suppresses the long period phenotype of a mutation in the clock gene period (per). NEJ physically interacts through two binding sites with CLOCK and the CLOCK. CYCLE (CLK.CYC) complex. Induction of CLK.CYC-dependent transcripts upon induction of nej expression from a heat-shock promoter showed that NEJ is limiting. Reduced CLK.CYC-mediated transcription in a nej hypomorphic mutant indicates an essential function of NEJ/CBP for CLK.CYC activity and a regulation of circadian transcription by availability of the co-activator. Competition for recruitment of NEJ/CBP provides a potential mechanism for cross-talk between circadian transcription and other CBP-dependent physiological processes. |
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Authors:
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Hsiu-Cheng Hung; Christian Maurer; Steve A Kay; Frank Weber |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-07-16 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 282 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-10-22 Completed Date: 2007-12-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 31349-57 Citation Subset: IM |
Affiliation:
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Biochemie-Zentrum Heidelberg, Ruprecht-Karls Universität Heidelberg, Im Neuenheimer Feld 328, 69120 Heidelberg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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ARNTL Transcription Factors Animals Basic Helix-Loop-Helix Transcription Factors / metabolism Binding Sites CLOCK Proteins Circadian Rhythm / genetics* Drosophila Drosophila Proteins / genetics, metabolism, physiology* Gene Expression Regulation* Genes, Reporter Immunohistochemistry Luciferases / metabolism Luminescent Measurements Promoter Regions, Genetic Protein Binding Reverse Transcriptase Polymerase Chain Reaction Trans-Activators / metabolism Transcription, Genetic* Transcriptional Activation* Transfection p300-CBP Transcription Factors / genetics, metabolism, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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MH51573/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/ARNTL Transcription Factors; 0/Basic Helix-Loop-Helix Transcription Factors; 0/CYCLE protein, Drosophila; 0/Drosophila Proteins; 0/Trans-Activators; 0/nejire protein, Drosophila; EC 1.13.12.-/Luciferases; EC 2.3.1.48/CLOCK Proteins; EC 2.3.1.48/p300-CBP Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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