| Circadian rhythmicity during prolonged chemostat cultivation of Neurospora crassa. | |
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MedLine Citation:
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PMID: 17196855 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Following exposure to light and attainment of steady-state in the chemostat, Neurospora was grown in constant conditions of darkness at 25 degrees C for 6 days. Biomass samples were taken every 4h for the extraction of RNA and protein, and the state of the circadian clock was assessed by assaying the levels of three rhythmically expressed mRNAs; frequency (frq), antisense frq (qrf) and clock-controlled gene-14 (ccg-14), and by monitoring the clock-controlled rhythm of sporulation. Our results indicate that the Neurospora clock continued to run in the chemostat. This is the longest reported time that Neurospora has been grown in a chemostat in filamentous form and opens up the possibility of studying the response of Neurospora to a range of stimuli in the absence of confounding effects due to; alterations in growth rate, aging, and changing conditions of the growth medium. |
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Authors:
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Tewes Tralau; Karin Lanthaler; Geoff D Robson; Susan K Crosthwaite |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-29 |
Journal Detail:
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Title: Fungal genetics and biology : FG & B Volume: 44 ISSN: 1087-1845 ISO Abbreviation: Fungal Genet. Biol. Publication Date: 2007 Aug |
Date Detail:
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Created Date: 2007-06-05 Completed Date: 2007-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9607601 Medline TA: Fungal Genet Biol Country: United States |
Other Details:
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Languages: eng Pagination: 754-63 Citation Subset: IM |
Affiliation:
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Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester M13 9PT, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Clocks Biomass Carbon Dioxide / metabolism Circadian Rhythm / physiology* Darkness Fungal Proteins / genetics Gene Expression Regulation, Fungal Light Microscopy, Electron, Transmission Neurospora crassa / chemistry, genetics, growth & development, physiology* RNA, Fungal / analysis, genetics RNA, Messenger / analysis, genetics Spores, Fungal / growth & development Temperature Time Factors |
| Chemical | |
Reg. No./Substance:
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0/FRQ protein, Neurospora crassa; 0/Fungal Proteins; 0/RNA, Fungal; 0/RNA, Messenger; 124-38-9/Carbon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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