Document Detail


Circadian measurements of sirtuin biology.
MedLine Citation:
PMID:  24014414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many of our behavioral and physiological processes display daily oscillations that are under the control of the circadian clock. The core molecular clock network is present in both the brain and peripheral tissues and is composed of a complex series of interlocking transcriptional/translational feedback loops that oscillate with a periodicity of ~24 h. Recent evidence has implicated NAD(+) biosynthesis and the sirtuin family of NAD(+)-dependent protein deacetylases as part of a novel feedback loop within the core clock network, findings which underscore the importance of taking circadian timing into consideration when designing and interpreting metabolic studies, particularly in regard to sirtuin biology. Thus, this chapter introduces both in vivo and in vitro circadian methods to analyze various sirtuin-related endpoints across the light-dark cycle and discusses the transcriptional, biochemical, and physiological outputs of the clock.
Authors:
Kathryn Moynihan Ramsey; Alison H Affinati; Clara B Peek; Biliana Marcheva; Hee-Kyung Hong; Joseph Bass
Related Documents :
2809704 - Across unit patterns in the neural response to taste: vector space analysis.
9622574 - Contralateral coordination and retargeting of limb movements during scratching in the l...
18425124 - Theta phase-specific codes for two-dimensional position, trajectory and heading in the ...
11410924 - Spontaneous motor unit behavior in human thenar muscles after spinal cord injury.
21395234 - General strategy for fabricating transparent tio(2) nanotube arrays for dye-sensitized ...
25347844 - Wing kinematics in a hovering dronefly minimize power expenditure.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  1077     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-09-09     Completed Date:  2014-04-03     Revised Date:  2014-07-11    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  285-302     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Circadian Clocks / physiology*
Circadian Rhythm / physiology*
Feedback, Physiological
Locomotion
Mice
Mice, Inbred C57BL
NAD / metabolism*
NIH 3T3 Cells
Sirtuins / genetics,  metabolism*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
F30 DK085936/DK/NIDDK NIH HHS; P01 AG011412/AG/NIA NIH HHS; R01 DK090625/DK/NIDDK NIH HHS; R01 HL097817/HL/NHLBI NIH HHS; T32 DK007169/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0U46U6E8UK/NAD; EC 3.5.1.-/Sirtuins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Methods to study the role of sirtuins in genome stability.
Next Document:  Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice.