Document Detail


Circadian clock regulation of the cell cycle in the zebrafish intestine.
MedLine Citation:
PMID:  24013905     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The circadian clock controls cell proliferation in a number of healthy tissues where cell renewal and regeneration are critical for normal physiological function. The intestine is an organ that typically undergoes regular cycles of cell division, differentiation and apoptosis as part of its role in digestion and nutrient absorption. The aim of this study was to explore circadian clock regulation of cell proliferation and cell cycle gene expression in the zebrafish intestine. Here we show that the zebrafish gut contains a directly light-entrainable circadian pacemaker, which regulates the daily timing of mitosis. Furthermore, this intestinal clock controls the expression of key cell cycle regulators, such as cdc2, wee1, p21, PCNA and cdk2, but only weakly influences cyclin B1, cyclin B2 and cyclin E1 expression. Interestingly, food deprivation has little impact on circadian clock function in the gut, but dramatically reduces cell proliferation, as well as cell cycle gene expression in this tissue. Timed feeding under constant dark conditions is able to drive rhythmic expression not only of circadian clock genes, but also of several cell cycle genes, suggesting that food can entrain the clock, as well as the cell cycle in the intestine. Rather surprisingly, we found that timed feeding is critical for high amplitude rhythms in cell cycle gene expression, even when zebrafish are maintained on a light-dark cycle. Together these results suggest that the intestinal clock integrates multiple rhythmic cues, including light and food, to function optimally.
Authors:
Elodie Peyric; Helen A Moore; David Whitmore
Related Documents :
23108335 - Immunological homeostasis of the eye.
24329715 - Intercellular trafficking of transcription factors in the vascular tissue patterning.
23992485 - Excess fructose intake-induced hypertrophic visceral adipose tissue results from unbala...
23163855 - Effects of tepary bean (phaseolus acutifolius) protease inhibitor and semipure lectin f...
11251185 - Rpr-115135, a new non peptidomimetic farnesyltransferase inhibitor, induces g0/g1 arres...
6693875 - Tetrahydrobiopterin and total biopterin content of neuroblastoma (n1e-115, n2a) and phe...
Publication Detail:
Type:  Journal Article     Date:  2013-08-27
Journal Detail:
Title:  PloS one     Volume:  8     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2013  
Date Detail:
Created Date:  2013-09-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e73209     Citation Subset:  IM    
Affiliation:
Centre for Cell and Molecular Dynamics, Department of Cell and Developmental Biology, University College London, London, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Phase II trial of capecitabine and everolimus (RAD001) combination in refractory gastric cancer pati...
Next Document:  Magnitude representations in williams syndrome: differential acuity in time, space and number proces...