| Cinobufacini induced MDA-MB-231 cell apoptosis-associated cell cycle arrest and cytoskeleton function. | |
| | |
MedLine Citation:
|
PMID: 22225634 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Cinobufacini is a traditional Chinese anti-tumor drug and widely used in clinic experiences. But little is known about its effect on the cells. In this study, the effects of cinobufacini on breast cancer MDA-MB-231 cell were evaluated by CCK-8 assay, and the data showed cinobufacini could inhibit the MDA-MB-231 cells growth effectively in dose-dependent and time-dependent manners. Cell apoptosis and cell cycle were detected by flow cytometry analysis. After the cells being treated with 50μg/mL cinobufacini for 48h, the early apoptosis percentage (20.45±1.46%) is much higher than the normal group (7.73±1.21%). The cell cycle data indicated that cinobufacini caused a cell cycle arrest at S phase. What's more, cinobufacini can affect the disruption of cytoskeleton, and these alterations changed the cell-surface ultrastructure and the cell morphology which were detected by atomic force microscopy (AFM) at nanoscale level. It indicated that the cell membrane structure and cytoskeleton networks were destroyed and the cell tails were narrowed after the cell being treated with cinobufacini. The present study is to provide valuable new insights to understand the mechanism of the drug in anti-tumor process. Furthermore, the knowledge concerning the signaling of cell cycle is potentially important to clinical utility. |
| | |
Authors:
|
Lina Ma; Bing Song; Hua Jin; Jiang Pi; Li Liu; Jinhuan Jiang; Jiye Cai |
Related Documents
:
|
21854034 - Efficient ternary blend bulk heterojunction solar cells with tunable open-circuit voltage. 22193304 - Comprehensive qpcr profiling of gene expression in single neuronal cells. 22199274 - Overexpressed her2 in nsclc is a possible therapeutic target of egfr inhibitors. 8032904 - Characterization of the effect of cholecystokinin (cck) on neurons in the periaqueducta... 5436084 - Colcemid sensitivity of fission yeast and the isolation of colcemid-resistant mutants. 14680934 - Expression of hepatitis b surface antigen in tobacco cell suspension cultures. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-30 |
Journal Detail:
|
Title: Bioorganic & medicinal chemistry letters Volume: - ISSN: 1464-3405 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2012-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
|
Department of Chemistry and Institute for Nano-Chemistry, Jinan University, Guangzhou 510632, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Design and synthesis of boronic acid inhibitors of endothelial lipase.
Next Document: Synthesis and biological evaluation of benzo[d]imidazole derivatives as potential anti-cancer agents...