| Ciliogenesis in normal human kidney development and post-natal life. | |
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MedLine Citation:
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PMID: 21688189 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Ciliogenesis in developing and post-natal human kidneys appears to influence cell proliferation and differentiation, apico-basal cell polarity, and tubular lumen formation. We have analyzed the appearance of primary cilia and differentiation of kidney cells in ten human conceptuses aged 6-22 weeks and in one 5-year-old kidney, using a double immunofluorescence labeling technique for α-tubulin, γ-tubulin, Oct-4, and Ki-67 and by electron microscopy. Immature forms of nephrons and ampullae were characterized by intense cell proliferation, which subsequently decreased during development. Primary cilia appeared on the surfaces of non-proliferating cells in developing nephrons, gradually increasing in length from 0.59 μm in renal vesicles to 0.81 μm in the S-forms of nephrons, ultimately reaching 3.04 μm in length in mature fetal and post-natal nephrons. Ciliary length increased from 0.59 μm in ampullae to 1.28 μm in post-natal collecting tubules. Mesenchymal to epithelial transformation of kidney cells coincided with the appearance of apico-basal polarity, both gap and tight junctions, and lumen formation. Up-regulation of Oct-4 expression correlated with the onset of kidney cell differentiation. Our results demonstrate the importance of proper primary cilia lengthening and Oct-4 expression for the normal development of fetal and post-natal kidneys and of apico-basal polarity for normal tubular lumen formation. Disturbances in these processes are associated with ciliopathies. |
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Authors:
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Mirna Saraga-Babić; Katarina Vukojević; Ivana Bočina; Kristina Drnašin; Marijan Saraga |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-19 |
Journal Detail:
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Title: Pediatric nephrology (Berlin, Germany) Volume: - ISSN: 1432-198X ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8708728 Medline TA: Pediatr Nephrol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Šoltanska 2, 21000, Split, Croatia, msb@mefst.hr. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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