Document Detail


Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis.
MedLine Citation:
PMID:  15790966     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cilia, as motile and sensory organelles, have been implicated in normal development, as well as diseases including cystic kidney disease, hydrocephalus and situs inversus. In kidney epithelia, cilia are proposed to be non-motile sensory organelles, while in the mouse node, two cilia populations, motile and non-motile have been proposed to regulate situs. We show that cilia in the zebrafish larval kidney, the spinal cord and Kupffer's vesicle are motile, suggesting that fluid flow is a common feature of each of these organs. Disruption of cilia structure or motility resulted in pronephric cyst formation, hydrocephalus and left-right asymmetry defects. The data show that loss of fluid flow leads to fluid accumulation, which can account for organ distension pathologies in the kidney and brain. In Kupffer's vesicle, loss of flow is associated with loss of left-right patterning, indicating that the 'nodal flow' mechanism of generating situs is conserved in non-mammalian vertebrates.
Authors:
Albrecht G Kramer-Zucker; Felix Olale; Courtney J Haycraft; Bradley K Yoder; Alexander F Schier; Iain A Drummond
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  132     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-25     Completed Date:  2005-06-21     Revised Date:  2014-02-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  1907-21     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Body Fluids / physiology*
Body Patterning / physiology
Central Nervous System / embryology*
Cilia / physiology*
Cloning, Molecular
DNA Primers
Dyneins / genetics
Embryo, Nonmammalian / embryology,  ultrastructure
Immunohistochemistry
In Situ Hybridization
Kidney / embryology*
Microscopy, Electron
Microscopy, Fluorescence
Microscopy, Video
Molecular Sequence Data
Nerve Tissue Proteins / genetics
Oligonucleotides
Organizers, Embryonic / embryology*
Organogenesis / physiology*
Sequence Analysis, DNA
Zebrafish / embryology*
Grant Support
ID/Acronym/Agency:
5RO1 GM56211/GM/NIGMS NIH HHS; DK53093/DK/NIDDK NIH HHS; DK54711/DK/NIDDK NIH HHS; DK65655/DK/NIDDK NIH HHS; T32 DK07545/DK/NIDDK NIH HHS; T32 GM007308/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Nerve Tissue Proteins; 0/Oligonucleotides; EC 3.6.4.2/Dyneins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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