| Cigarette smoking increases aortic dilatation without affecting matrix metalloproteinase-9 and -12 expression in a modified mouse model of aneurysm formation. | |
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MedLine Citation:
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PMID: 17398058 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The development of abdominal aortic aneurysms (AAA) is presumed to result from multiple genetic and environmental factors, with exposure to tobacco smoke the single largest known factor predisposing to aneurysm growth. We have attempted to adapt the elastase-perfused animal model to determine whether tobacco exposure can lower the threshold of aortic injury necessary for AAA development. METHODS: Adult C57BL/6 mice underwent transient perfusion of the infrarenal aorta with an active solution of elastase: high-dose (HDE, 0.19 U/mL, n=9), standard-dose (SDE, 0.16 U/mL, n=21) or low-dose (LDE, 0.07 U/mL, n=24). Control animals (n=24) were treated with heat inactivated elastase (HIE). Twenty LDE perfused mice were exposed to cigarette smoke (LDE-S) beginning 2 weeks before perfusion and continuing until aortic harvest. Aortic diameter (AD) was measured preperfusion, postperfusion, and at harvest on day 14. AAA was defined as %DeltaAD>or=100% between preperfusion and harvest. Aortas from each group (except HDE) were analyzed for matrix metalloproteinase-9 (MMP-9) and MMP-12 expression by real-time polymerase chain reaction normalized to glyceraldehyde-3-phosphate dehydrogenase. RESULTS: All SDE mice developed large AAA by %DeltaAD (189.3%+/-16.9%, mean+/-standard error of the mean), but control mice had only a small dilatation (69.7%+/-3.7%, P<.01). Higher doses of elastase did not produce larger aneurysms in HDE mice. In contrast, only 63% of LDE mice showed aneurysmal dilatation, and these were significantly smaller (104.3%+/-4.2%, P<.01). When exposed to cigarette smoke, LDE animals developed significantly larger aneurysms (%DeltaAD, 134.5%+/-7.9%, P=.0021). There was no difference in normalized aortic MMP-9 and MMP-12 expression between elastase doses or between smoke-exposed and unexposed animals. Histologic analysis revealed that smoking increased the extent of aortic elastin degradation when compared with LDE-S animals. CONCLUSION: Aneurysm development in the elastase model is dependent on the quantity of active elastase infused. Exposure of animals to tobacco smoke after a relatively minor aortic elastase injury produces increases in elastin degradation and aneurysm size without affecting MMP-9 or MMP-12 expression. To our knowledge, this is the first demonstration in an animal model that smoking can act as a synergistic factor in AAA development. Further understanding of the relationship between smoking and AAA in this model may help unveil the pathophysiologic pathways involved between cigarette smoke and AAAs. |
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Authors:
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Michel P Bergoeing; Batool Arif; Amy E Hackmann; Terri L Ennis; Robert W Thompson; John A Curci |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-03-29 |
Journal Detail:
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Title: Journal of vascular surgery Volume: 45 ISSN: 0741-5214 ISO Abbreviation: J. Vasc. Surg. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-06-04 Completed Date: 2007-07-13 Revised Date: 2012-10-03 |
Medline Journal Info:
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Nlm Unique ID: 8407742 Medline TA: J Vasc Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1217-1227 Citation Subset: IM |
Affiliation:
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Department of Surgery (Section of Vascular Surgery), Washington University School of Medicine, St. Louis, MO 63110, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta, Abdominal / drug effects*, enzymology, pathology Aortic Aneurysm, Abdominal / chemically induced, etiology*, metabolism, pathology, physiopathology Blood Pressure / drug effects Cotinine / urine Dilatation, Pathologic Disease Models, Animal Dose-Response Relationship, Drug Gene Expression Regulation, Enzymologic / drug effects Male Matrix Metalloproteinase 12 / analysis*, genetics Matrix Metalloproteinase 9 / analysis*, genetics Mice Mice, Inbred C57BL Pancreatic Elastase RNA, Messenger / analysis Risk Factors Smoke / adverse effects* Time Factors Tobacco* |
| Grant Support | |
ID/Acronym/Agency:
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DK56341/DK/NIDDK NIH HHS; P30 DK056341-06/DK/NIDDK NIH HHS; P30 DK056341-07/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/Smoke; 486-56-6/Cotinine; EC 3.4.21.36/Pancreatic Elastase; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 3.4.24.65/Matrix Metalloproteinase 12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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