| Cigarette smoke inhibits endometrial epithelial cell proliferation through a nitric oxide-mediated pathway. | |
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MedLine Citation:
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PMID: 19022425 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine the direct effects of cigarette smoke (CS) on human endometrial surface epithelial cell line proliferation. DESIGN: In vitro study using HES cells and primary human endometrial cells. SETTING: University-based academic center. PATIENT(S): Premenopausal women in the proliferative phase of the cycle. INTERVENTION(S): The HES cells and primary human endometrial cells were exposed to cigarette smoke-saturated solution. MAIN OUTCOME MEASURE(S): Cell proliferation and expression of different isoforms of nitric oxide synthase. RESULT(S): Cigarette smoke inhibited HES cell proliferation in a dose- and time-dependant manner. The inhibitory effect of CS was blocked by hemoglobin and enhanced by L-arginine (L-Arg). Cigarette smoking and nicotine stimulated the expression of endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) whereas benzo[a]pyrene (BP) only stimulated the expression of eNOS in HES cells. Cigarette smoke stimulated the expression of eNOS/iNOS in primary human endometrial cells, comprised of epithelial and stromal cells. The effect of CS on eNOS/iNOS expression in HES cells was blocked by ascorbic acid but not by glutathione. CONCLUSION(S): Cigarette smoke inhibits endometrial cell proliferation through a nitric oxide-mediated pathway. |
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Authors:
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Omid Khorram; Guang Han; Thomas Magee |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-11-19 |
Journal Detail:
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Title: Fertility and sterility Volume: 93 ISSN: 1556-5653 ISO Abbreviation: Fertil. Steril. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-27 Completed Date: 2010-02-22 Revised Date: 2011-10-27 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: United States |
Other Details:
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Languages: eng Pagination: 257-63 Citation Subset: IM |
Copyright Information:
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Copyright 2010. Published by Elsevier Inc. |
Affiliation:
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Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, California, USA. okhorram@obgyn.humc.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antioxidants
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pharmacology Arginine / metabolism Ascorbic Acid / pharmacology Benzo(a)pyrene / toxicity Cell Proliferation / drug effects* Cells, Cultured Dose-Response Relationship, Drug Endometrium / drug effects*, metabolism, pathology Epithelial Cells / drug effects*, metabolism, pathology Female Follicular Phase Glutathione / metabolism Hemoglobins / metabolism Humans Nicotine / toxicity Nicotinic Agonists / toxicity Nitric Oxide / metabolism* Nitric Oxide Synthase Type II / metabolism Nitric Oxide Synthase Type III / metabolism Premenopause Signal Transduction / drug effects* Smoke / adverse effects* Time Factors Tobacco* |
| Grant Support | |
ID/Acronym/Agency:
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R03 41409-01//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Hemoglobins; 0/Nicotinic Agonists; 0/Smoke; 10102-43-9/Nitric Oxide; 50-32-8/Benzo(a)pyrene; 50-81-7/Ascorbic Acid; 54-11-5/Nicotine; 70-18-8/Glutathione; 74-79-3/Arginine; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/NOS3 protein, human; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nitric Oxide Synthase Type III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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