Document Detail

Cigarette smoke inhibits endometrial epithelial cell proliferation through a nitric oxide-mediated pathway.
MedLine Citation:
PMID:  19022425     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine the direct effects of cigarette smoke (CS) on human endometrial surface epithelial cell line proliferation.
DESIGN: In vitro study using HES cells and primary human endometrial cells.
SETTING: University-based academic center.
PATIENT(S): Premenopausal women in the proliferative phase of the cycle.
INTERVENTION(S): The HES cells and primary human endometrial cells were exposed to cigarette smoke-saturated solution.
MAIN OUTCOME MEASURE(S): Cell proliferation and expression of different isoforms of nitric oxide synthase.
RESULT(S): Cigarette smoke inhibited HES cell proliferation in a dose- and time-dependant manner. The inhibitory effect of CS was blocked by hemoglobin and enhanced by L-arginine (L-Arg). Cigarette smoking and nicotine stimulated the expression of endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) whereas benzo[a]pyrene (BP) only stimulated the expression of eNOS in HES cells. Cigarette smoke stimulated the expression of eNOS/iNOS in primary human endometrial cells, comprised of epithelial and stromal cells. The effect of CS on eNOS/iNOS expression in HES cells was blocked by ascorbic acid but not by glutathione.
CONCLUSION(S): Cigarette smoke inhibits endometrial cell proliferation through a nitric oxide-mediated pathway.
Omid Khorram; Guang Han; Thomas Magee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-11-19
Journal Detail:
Title:  Fertility and sterility     Volume:  93     ISSN:  1556-5653     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-02-22     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  United States    
Other Details:
Languages:  eng     Pagination:  257-63     Citation Subset:  IM    
Copyright Information:
Copyright 2010. Published by Elsevier Inc.
Department of Obstetrics and Gynecology, Harbor-UCLA Medical Center, Torrance, California, USA.
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MeSH Terms
Antioxidants / pharmacology
Arginine / metabolism
Ascorbic Acid / pharmacology
Benzo(a)pyrene / toxicity
Cell Proliferation / drug effects*
Cells, Cultured
Dose-Response Relationship, Drug
Endometrium / drug effects*,  metabolism,  pathology
Epithelial Cells / drug effects*,  metabolism,  pathology
Follicular Phase
Glutathione / metabolism
Hemoglobins / metabolism
Nicotine / toxicity
Nicotinic Agonists / toxicity
Nitric Oxide / metabolism*
Nitric Oxide Synthase Type II / metabolism
Nitric Oxide Synthase Type III / metabolism
Signal Transduction / drug effects*
Smoke / adverse effects*
Time Factors
Grant Support
R03 41409-01//PHS HHS
Reg. No./Substance:
0/Antioxidants; 0/Hemoglobins; 0/Nicotinic Agonists; 0/Smoke; 10102-43-9/Nitric Oxide; 50-32-8/Benzo(a)pyrene; 50-81-7/Ascorbic Acid; 54-11-5/Nicotine; 70-18-8/Glutathione; 74-79-3/Arginine; EC protein, human; EC protein, human; EC Oxide Synthase Type II; EC Oxide Synthase Type III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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