| Cigarette smoke induces rapid changes in gene expression in pulmonary arteries. | |
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MedLine Citation:
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PMID: 12379773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The pathogenesis of cigarette smoke-induced pulmonary hypertension is not well characterized. We used RT-PCR to examine gene expression of nitric oxide synthase 2 (NOS-2), nitric oxide synthase 3 (NOS-3), endothelin, and vascular endothelial growth factor (VEGF) and its flk-1 receptor (VEGF-R) in main pulmonary arteries and in intraparenchymal arteries microdissected from alcohol-fixed paraffin blocks. The main pulmonary artery and intraparenchymal vessels responded in a similar fashion, with up-regulation of endothelin, VEGF, and VEGF-R gene expression evident by 2 hours after smoke exposure. Up-regulation of gene expression was still present at 24 hours after exposure, and at this time there was also a small increase in NOS-2. As a comparison, we examined the trachea and microdissected intraparenchymal airways and found up-regulation of endothelin and NOS-2 at 2 hours and additional up-regulation of NOS-3 at 24 hours. These findings suggest that the pulmonary vasculature very rapidly responds to cigarette smoke with up-regulation of mediators that control vascular cell proliferation and vascular constriction. These changes support the idea that pulmonary hypertension in cigarette smokers reflects a direct effect of smoke on the vasculature. The pattern of response in the vessels is distinctly different from that in the airways. |
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Authors:
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Joanne L Wright; Hsin Tai; Jin Dai; Andrew Churg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 82 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2002-10-15 Completed Date: 2002-11-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: United States |
Other Details:
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Languages: eng Pagination: 1391-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of British Columbia, Vancouver, British Columbia, Canada. jlwright@interchange.ubc.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Gene Expression Regulation* Hypertension, Pulmonary / etiology* Muscle, Smooth, Vascular / pathology Pulmonary Artery / pathology* Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Smoke / adverse effects* Smoking / adverse effects* |
| Chemical | |
Reg. No./Substance:
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0/Smoke |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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