Document Detail

Cigarette smoke increases BLT2 receptor functions in bronchial epithelial cells: in vitro and ex vivo evidence.
MedLine Citation:
PMID:  23347335     Owner:  NLM     Status:  MEDLINE    
Leukotriene B(4) (LTB(4)) is a neutrophil chemotactic molecule with important involvement in the inflammatory responses of chronic obstructive pulmonary disease (COPD). Airway epithelium is emerging as a regulator of innate immune responses to a variety of insults including cigarette smoke, the major risk factor for COPD. In this study we have explored whether cigarette smoke extracts (CSE) or soluble mediators present in distal lung fluid samples (mini-bronchoalveolar lavages) from smokers alter the expression of the LTB(4) receptor 2 (BLT2) and peroxisome proliferator-activated receptor-α (PPAR-α) in bronchial epithelial cells. We also evaluated the effects of CSE on the expression of intercellular adhesion molecule 1 (ICAM-1) and on the binding of signal transducer and activator of transcription 1 (STAT-1) to ICAM-1 promoter as well as the adhesiveness of neutrophils to bronchial epithelial cells. CSE and mini-bronchoalveolar lavages from smokers increased BLT2 and ICAM-1 expression as well as the adhesiveness of neutrophils to bronchial epithelial cells and decreased PPAR-α expression. CSE induced the activation of STAT-1 and its binding to ICAM-1 promoter. These findings suggest that, in bronchial epithelial cells, CSE promote a prevalent induction of pro-inflammatory BLT2 receptors and activate mechanisms leading to increased neutrophil adhesion, a mechanism that contributes to airway neutrophilia and to tissue damage.
Elisabetta Pace; Maria Ferraro; Serena Di Vincenzo; Andreina Bruno; Antonino Giarratano; Valeria Scafidi; Luana Lipari; Denise Valentina Di Benedetto; Serafina Sciarrino; Mark Gjomarkaj
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  139     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-04-24     Completed Date:  2013-07-01     Revised Date:  2014-06-03    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  245-55     Citation Subset:  IM    
Copyright Information:
© 2013 Blackwell Publishing Ltd.
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MeSH Terms
Blotting, Western
Bronchi / cytology,  immunology,  metabolism
Bronchoalveolar Lavage Fluid / chemistry,  immunology
Cell Adhesion / drug effects,  immunology
Cells, Cultured
Epithelial Cells / drug effects,  immunology*,  metabolism
Flow Cytometry
Intercellular Adhesion Molecule-1 / genetics,  immunology,  metabolism
Neutrophils / cytology,  immunology,  metabolism
PPAR alpha / genetics,  immunology,  metabolism
Plant Extracts / immunology,  metabolism,  pharmacology
Promoter Regions, Genetic / genetics
Protein Binding / drug effects,  immunology
RNA Interference
Receptors, Leukotriene B4 / genetics,  immunology*,  metabolism
STAT1 Transcription Factor / immunology,  metabolism
Smoking / immunology*,  metabolism
Tobacco / chemistry
Reg. No./Substance:
0/LTB4R2 protein, human; 0/PPAR alpha; 0/Plant Extracts; 0/Receptors, Leukotriene B4; 0/STAT1 Transcription Factor; 126547-89-5/Intercellular Adhesion Molecule-1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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