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Cigarette smoke extract inhibits the proliferation of alveolar epithelial cells and induces apoptosis.
MedLine Citation:
PMID:  16786109     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Cigarette smoke extract (CSE) contains abundant oxidants and free radicals. Oxidative stress caused by cigarette smoking results in the destruction of the alveolar cell walls and emphysema. However, there exists discrepancy about how CSE works in the process. In the present study, we observed the effect of CSE on the cell growth of type II alveolar epithelial cell-derived A549 cell line, and provided molecular understanding of this effect. The MTT assay results showed that CSE decreased the cell viability of A549 cells in a dose- and time-dependent manner, and cell cycle was arrested in G(1)/S phase. Furthermore, CSE-induced apoptosis of A549 cells was verified by Hoechst 33258 staining, electron microscopy in morphology, and the appearance of DNA fragmentation and annexin V-FITC/propidium iodide (PI) staining assay at molecular level. It was found that CSE treatment resulted in the upregulation of Fas/APO-1 receptor and activation of caspase-3. CSE also initiated accumulation of intracellular reactive oxygen species, which was detected by laser confocal microscopy. Taken together, CSE could inhibit the cell growth and induce apoptosis of A549 cells through Fas receptor pathway. Oxidative stress caused by CSE may be the radical factor leading to apoptosis as well as cell growth inhibition in alveolar epithelial cells.
Authors:
Zong-Xian Jiao; Qi-Lin Ao; Mi Xiong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Sheng li xue bao : [Acta physiologica Sinica]     Volume:  58     ISSN:  0371-0874     ISO Abbreviation:  Sheng Li Xue Bao     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  20730130R     Medline TA:  Sheng Li Xue Bao     Country:  China    
Other Details:
Languages:  eng     Pagination:  244-54     Citation Subset:  IM    
Affiliation:
Department of Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. E-mail: aoqilin@263.net.
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