| Cigarette smoke extract induces HO-1 expression in mouse cerebral vascular endothelial cells: involvement of c-Src/NADPH oxidase/PDGFR/JAK2/STAT3 pathway. | |
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MedLine Citation:
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PMID: 20568122 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Several chemicals present in cigarette smoke (CS) have been reported to induce heme oxygenase-1 (HO-1) expression, which represents a prime defense mechanism in protecting the cells from stress-dependent adverse effects on peripheral vascular system. However, the effects of cigarette smoke extract (CSE) on HO-1 induction and the mechanisms underlying CSE-induced HO-1 expression in brain vessels are not completely understood. Here, we used a mouse brain endothelial cell culture (bEnd.3) to investigate the effect of CSE on HO-1 induction and the mechanisms underlying CSE-induced HO-1 expression in cerebral vessels. We demonstrated that sublethal concentrations of CSE (30 µg/ml) induced submaximal HO-1 expression in bEnd.3 cells. NADPH oxidase-dependent ROS generation played a key role in CSE-induced HO-1 expression. CSE-induced HO-1 expression was mediated through PDGFR/JAK2/STAT3 cascade, which was observed by pretreatment with the respective pharmacological inhibitors or transfection with PDGFR shRNA. CSE activated NADPH oxidase through c-Src in bEnd.3 cells. Taken together, these results suggested that, in bEnd.3 cells, CSE-induced HO-1 expression was mediated through PDGFR/JAK2/STAT3 cascade, which was regulated by c-Src or c-Src activated-NADPH oxidase/ROS. |
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Authors:
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Ruey-Horng Shih; I-Ta Lee; Hsi-Lung Hsieh; Yu Ru Kou; Chuen-Mao Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cellular physiology Volume: 225 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2010-10-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 741-50 Citation Subset: IM |
Copyright Information:
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© 2010 Wiley-Liss, Inc. |
Affiliation:
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Department of Physiology and Pharmacology, Chang Gung University, Tao-Yuan, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding Sites Brain / blood supply* Cells, Cultured Endothelial Cells / drug effects*, enzymology Enzyme Induction Enzyme Inhibitors / pharmacology Free Radical Scavengers / pharmacology Heme Oxygenase-1 / biosynthesis*, genetics Janus Kinase 2 / metabolism* Membrane Proteins / biosynthesis*, genetics Mice NADPH Oxidase / metabolism* Promoter Regions, Genetic RNA Interference RNA, Messenger / biosynthesis Reactive Oxygen Species / metabolism Receptors, Platelet-Derived Growth Factor / genetics, metabolism* STAT3 Transcription Factor / metabolism* Signal Transduction / drug effects Smoke* Smoking / adverse effects* Time Factors src-Family Kinases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Free Radical Scavengers; 0/Membrane Proteins; 0/RNA, Messenger; 0/Reactive Oxygen Species; 0/STAT3 Transcription Factor; 0/Smoke; 0/Stat3 protein, mouse; EC 1.14.99.3/Heme Oxygenase-1; EC 1.14.99.3/Hmox1 protein, mouse; EC 1.6.3.1/NADPH Oxidase; EC 2.7.1.112/Jak2 protein, mouse; EC 2.7.10.1/Janus Kinase 2; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor; EC 2.7.10.2/src-Family Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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