| Cigarette smoke extract increases S-adenosylmethionine and cystathionine in human lung epithelial-like (A549) cells. | |
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MedLine Citation:
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PMID: 14726155 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of cigarette smoke extract (CSE) on S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and sulfur amino acid metabolism was examined in human lung epithelial-like (A549) cells exposed to various CSE concentrations (2.5-100%) for 24 or 48 h. Intracellular SAM and SAM/SAH ratio were elevated after exposure to CSE for 48 h. Cell SAH content decreased, but the effect was not consistent. Cellular cystathionine, cysteine, and methionine levels were increased after CSE exposure for 48h. Sub-acute exposure to CSE induced increases in cellular SAM and SAM/SAH ratio. The transsulfuration pathway was likely activated by CSE since cystathionine increased, potentially contributing to the increased total intracellular GSH content. |
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Authors:
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Mihalis I Panayiotidis; Sally P Stabler; Robert H Allen; Aftab Ahmad; Carl W White |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Chemico-biological interactions Volume: 147 ISSN: 0009-2797 ISO Abbreviation: Chem. Biol. Interact. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-01-16 Completed Date: 2004-03-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0227276 Medline TA: Chem Biol Interact Country: Ireland |
Other Details:
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Languages: eng Pagination: 87-97 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids, Sulfur
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metabolism Carcinoma / metabolism, pathology Cell Line, Tumor Cell Survival / drug effects Cystathionine / metabolism* Cysteine / metabolism DNA / analysis Glutathione / analysis Humans Lung Neoplasms / metabolism*, pathology Methionine / metabolism S-Adenosylhomocysteine / metabolism S-Adenosylmethionine / metabolism* Smoke* Time Factors Tobacco* |
| Grant Support | |
ID/Acronym/Agency:
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AG 09834/AG/NIA NIH HHS; HL 52732/HL/NHLBI NIH HHS; HL 56263/HL/NHLBI NIH HHS; HL 57144/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids, Sulfur; 0/Smoke; 29908-03-0/S-Adenosylmethionine; 52-90-4/Cysteine; 56-88-2/Cystathionine; 63-68-3/Methionine; 70-18-8/Glutathione; 9007-49-2/DNA; 979-92-0/S-Adenosylhomocysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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