| Cigarette smoke decreases innate responses of epithelial cells to rhinovirus infection. | |
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MedLine Citation:
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PMID: 20224072 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exposure to cigarette smoke is associated with a significant increase in the risk for respiratory viral infections. The airway epithelium is the primary target for both cigarette smoke and respiratory viral infection. We investigated the effects of cigarette smoke on the response of airway epithelial cells to rhinovirus infection. We found that pre-exposure of BEAS-2B cells or primary normal human bronchial epithelial cells (NHBEs) to cigarette smoke extract (CSE) reduced the induction of mRNA of the chemokines CXCL10 and CCL5 by either the viral mimic polyinosine-polycytidylic acid (Poly I:C) or human rhinovirus 16 (HRV-16) infection. The HRV-16-induced release of CXCL10 and CCL5 was also significantly suppressed by CSE. Activation of the IFN mediator STAT-1 and the activation of JNK by poly I:C and HRV-16 were partially suppressed by pre-exposure to CSE. In contrast, the poly I:C-induced and HRV-16-induced phosphorylation of ERK1/2 was unaffected by CSE. HRV-16-stimulated IFN-β mRNA was also significantly reduced by CSE. Because suppression of the IFN response to viral infection was associated with increased viral production, we assessed HRV-16 RNA concentrations. Exposure to CSE resulted in an increase in HRV-16 RNA at 48 hours after the infection of BEAS-2B cells. These data demonstrate that exposure to CSE alters the response of airway epithelial cells to HRV infection, leading to decreased activation of the IFN-STAT-1 and SAP-JNK pathways, the suppression of CXCL10 and CCL5 production, and increased viral RNA. A diminished, early epithelial-initiated antiviral response to rhinovirus infection could contribute to the increased susceptibility of subjects to prolonged respiratory viral infections after exposure to cigarette smoke. |
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Authors:
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Jane Eddleston; Rachel U Lee; Astrid M Doerner; Jack Herschbach; Bruce L Zuraw |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-03-11 |
Journal Detail:
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Title: American journal of respiratory cell and molecular biology Volume: 44 ISSN: 1535-4989 ISO Abbreviation: Am. J. Respir. Cell Mol. Biol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-24 Completed Date: 2011-01-20 Revised Date: 2012-01-02 |
Medline Journal Info:
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Nlm Unique ID: 8917225 Medline TA: Am J Respir Cell Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 118-26 Citation Subset: IM |
Affiliation:
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Veterans Medical Research Foundation, and Department of Medicine, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0732, USA. jeddleston@vapop.ucsd.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Chemokine CCL5 / genetics, metabolism Chemokine CXCL10 / genetics, metabolism Dose-Response Relationship, Drug Down-Regulation Enzyme Activation Epithelial Cells / drug effects*, enzymology, immunology, virology Humans Immunity, Innate / drug effects* Interferon Regulatory Factor-3 / metabolism Interferon-beta / genetics, metabolism JNK Mitogen-Activated Protein Kinases / metabolism Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Phosphorylation Poly I-C / immunology RNA, Messenger / metabolism RNA, Viral / biosynthesis Respiratory Mucosa / drug effects*, enzymology, immunology, virology Rhinovirus / genetics, growth & development, immunology* STAT1 Transcription Factor / metabolism Smoke / adverse effects* Smoking / adverse effects* Time Factors Viral Load Virus Replication |
| Grant Support | |
ID/Acronym/Agency:
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AI50498/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CCL5 protein, human; 0/CXCL10 protein, human; 0/Chemokine CCL5; 0/Chemokine CXCL10; 0/IRF3 protein, human; 0/Interferon Regulatory Factor-3; 0/RNA, Messenger; 0/RNA, Viral; 0/STAT1 Transcription Factor; 0/STAT1 protein, human; 0/Smoke; 24939-03-5/Poly I-C; 77238-31-4/Interferon-beta; EC 2.7.11.24/JNK Mitogen-Activated Protein Kinases; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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