Document Detail


Chylomicron metabolism by the isolated perfused mouse heart.
MedLine Citation:
PMID:  11440913     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The catabolism of rat chylomicrons, labeled in their triacylglycerol (TG) component, was investigated using perfused working mouse hearts. Perfusion of mouse hearts with heparin increased lipoprotein lipase (LPL) activity in the perfusate. This heparin-releasable LPL pool remained constant over a variety of experimental conditions, including workload and fatty acid concentrations, making the mouse heart a suitable model to study chylomicron catabolism. Endothelium-bound LPL hydrolyzed radiolabeled (3)H-labeled chylomicrons (0.4 mM TG); the fate of LPL-derived (3)H-labeled fatty acids was split evenly between oxidation (production of (3)H(2)O) and esterification (incorporation into tissue lipids, mainly TG). In comparison, the oxidation of 0.4 mM [(3)H]palmitate complexed to albumin was fourfold greater than esterification into tissue lipids. Surprisingly, the addition of unlabeled palmitate (0.4 or 1.2 mM) to perfusions with (3)H-chylomicrons did not affect the fate (either oxidation or esterification) of LPL-derived (3)H-fatty acids. These results suggest that fatty acids produced from lipoprotein hydrolysis by the action of LPL and fatty acids from a fatty acid-albumin complex do not enter a common metabolic pool in the heart.
Authors:
K Mardy; D D Belke; D L Severson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  281     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-07-06     Completed Date:  2001-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E357-64     Citation Subset:  IM    
Affiliation:
Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chylomicrons / metabolism*,  pharmacology
Dose-Response Relationship, Drug
Endothelium, Vascular / metabolism
Enzyme Activation / drug effects
Esterification / drug effects
Heart / drug effects
Heparin / pharmacology
Hydrolysis
Lipoprotein Lipase / metabolism
Macromolecular Substances
Male
Mice
Models, Cardiovascular
Myocardial Contraction / physiology
Myocardium / metabolism*
Oxidation-Reduction / drug effects
Palmitic Acid / metabolism,  pharmacology
Perfusion
Rats
Serum Albumin / metabolism
Tritium
Chemical
Reg. No./Substance:
0/Chylomicrons; 0/Macromolecular Substances; 0/Serum Albumin; 10028-17-8/Tritium; 57-10-3/Palmitic Acid; 9005-49-6/Heparin; EC 3.1.1.34/Lipoprotein Lipase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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