Document Detail


Chylomicron- and VLDL-derived lipids enter the heart through different pathways: in vivo evidence for receptor- and non-receptor-mediated fatty acid uptake.
MedLine Citation:
PMID:  20852327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipids circulate in the blood in association with plasma lipoproteins and enter the tissues either after hydrolysis or as non-hydrolyzable lipid esters. We studied cardiac lipids, lipoprotein lipid uptake, and gene expression in heart-specific lipoprotein lipase (LpL) knock-out (hLpL0), CD36 knock-out (Cd36(-/-)), and double knock-out (hLpL0/Cd36(-/-)-DKO) mice. Loss of either LpL or CD36 led to a significant reduction in heart total fatty acyl-CoA (control, 99.5 ± 3.8; hLpL0, 36.2 ± 3.5; Cd36(-/-), 57.7 ± 5.5 nmol/g, p < 0.05) and an additive effect was observed in the DKO (20.2 ± 1.4 nmol/g, p < 0.05). Myocardial VLDL-triglyceride (TG) uptake was reduced in the hLpL0 (31 ± 6%) and Cd36(-/-) (47 ± 4%) mice with an additive reduction in the DKO (64 ± 5%) compared with control. However, LpL but not CD36 deficiency decreased VLDL-cholesteryl ester uptake. Endogenously labeled mouse chylomicrons were produced by tamoxifen treatment of β-actin-MerCreMer/LpL(flox/flox) mice. Induced loss of LpL increased TG levels >10-fold and reduced HDL by >50%. After injection of these labeled chylomicrons in the different mice, chylomicron TG uptake was reduced by ∼70% and retinyl ester by ∼50% in hLpL0 hearts. Loss of CD36 did not alter either chylomicron TG or retinyl ester uptake. LpL loss did not affect uptake of remnant lipoproteins from ApoE knock-out mice. Our data are consistent with two pathways for fatty acid uptake; a CD36 process for VLDL-derived fatty acid and a non-CD36 process for chylomicron-derived fatty acid uptake. In addition, our data show that lipolysis is involved in uptake of core lipids from TG-rich lipoproteins.
Authors:
Kalyani G Bharadwaj; Yaeko Hiyama; Yunying Hu; Lesley Ann Huggins; Rajasekhar Ramakrishnan; Nada A Abumrad; Gerald I Shulman; William S Blaner; Ira J Goldberg
Related Documents :
15530147 - Chemical-physical properties of lipoproteins in anorexia nervosa.
1596517 - The effects of season and exercise on the levels of plasma polyunsaturated fatty acids ...
1543697 - Lipoprotein-proteoglycan complexes from atherosclerotic lesions promote cholesteryl est...
2004437 - Further insights into the pathophysiology of hyperapobetalipoproteinemia: role of basic...
8238877 - Analysis of femtomole concentrations of alpha-ketoisocaproic acid in brain tissue by pr...
6388567 - Proteolytic conversion of insulin-like growth factors to an acidic form(s).
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-18
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  285     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-29     Completed Date:  2010-12-30     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37976-86     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD36 / genetics,  metabolism*
Antineoplastic Agents, Hormonal / pharmacokinetics
Cholesterol, VLDL / genetics,  metabolism*
Chylomicrons / genetics,  metabolism*
Fatty Acids / genetics,  metabolism*
Lipid Metabolism / drug effects,  physiology*
Lipoprotein Lipase / genetics,  metabolism*
Lipoproteins, VLDL / genetics,  metabolism*
Mice
Mice, Knockout
Myocardium / metabolism*
Tamoxifen / pharmacology
Triglycerides / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
AA019413/AA/NIAAA NIH HHS; DK079221/DK/NIDDK NIH HHS; HL45095/HL/NHLBI NIH HHS; HL73029/HL/NHLBI NIH HHS; P01 HL057278/HL/NHLBI NIH HHS; P30 DK034989/DK/NIDDK NIH HHS; P30 DK056341/DK/NIDDK NIH HHS; P30 DK056341-10/DK/NIDDK NIH HHS; R01 DK033301/DK/NIDDK NIH HHS; R01 DK040936/DK/NIDDK NIH HHS; R01 HL045095/HL/NHLBI NIH HHS; R01 HL073029/HL/NHLBI NIH HHS; U24 DK059635/DK/NIDDK NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Antineoplastic Agents, Hormonal; 0/Cholesterol, VLDL; 0/Chylomicrons; 0/Fatty Acids; 0/Lipoproteins, VLDL; 0/Triglycerides; 0/very low density lipoprotein triglyceride; 094ZI81Y45/Tamoxifen; EC 3.1.1.34/Lipoprotein Lipase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The association between home smoking restrictions and youth smoking behaviour: a review.
Next Document:  Right atrial lipoma in patient with Cowden syndrome.