Document Detail

Chrysotile-induced cell transformation and transcriptional changes of c-myc oncogene in human embryo lung cells.
MedLine Citation:
PMID:  11198217     Owner:  NLM     Status:  MEDLINE    
It has been verified that asbestos induce neoplastic transformation of several rodent cell lines in vitro. Very little information is available, however, on asbestos-induced human cell line transformation and oncogene activation in the development of asbestos-induced cell transformation. This study was designed to investigate the induction of cell transformation and transcriptional changes of the c-myc oncogene by chrysotile asbestos fibers (CAF) in human embryo lung (HEL) cells. Surface-modified CAF soaked in aluminium citrate (AC), rare earth compounds (REC) or sodium selenite (SS) solutions were used to observe the effects of surface modification on chrysotile-induced cell transformation and transcriptional changes in the c-myc oncogene. The results showed that the natural CAF caused dose-dependent increases in transformed foci, along with increased transcription of the c-myc oncogene in HEL cells, while the pretreated CAF induced fewer transformed foci and less c-myc oncogene transcription than the natural CAF. The percent inhibition of equivalent AC, REC and SS were 38.9%, 50.0%, 33.3% (for transformation focus), 81.6%, 69.5%, 89.9% (for c-myc oncogene transcription), respectively. These results indicated that CAF may induce cell transformation and transcriptional changes and that the carcinogenicity of CAF might be reduced by pretreatment of CAF with the above-mentioned compounds.
J G Fan; Q E Wang; S J Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical and environmental sciences : BES     Volume:  13     ISSN:  0895-3988     ISO Abbreviation:  Biomed. Environ. Sci.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2001-01-24     Completed Date:  2001-04-19     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8909524     Medline TA:  Biomed Environ Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  163-9     Citation Subset:  IM    
National Center for Occupational Safety Science and Technology, Beijing 100029, China.
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MeSH Terms
Asbestos, Serpentine / pharmacology*
Carcinogens / pharmacology*
Cell Transformation, Neoplastic / drug effects*
Genes, myc*
Lung / cytology,  drug effects*,  embryology
Transcription, Genetic / drug effects*
Reg. No./Substance:
0/Asbestos, Serpentine; 0/Carcinogens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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