Document Detail

Chrysosporium lucknowense arabinohydrolases effectively degrade sugar beet arabinan.
MedLine Citation:
PMID:  20566287     Owner:  NLM     Status:  MEDLINE    
The filamentous fungus Chrysosporium lucknowense (C1) is a rich source of cell wall degrading enzymes. In the present paper four arabinose releasing enzymes from C1 were characterized, among them one endoarabinanase, two arabinofuranosidases and one exoarabinanase. Combinations of these enzymes released up to 80% of the arabinose present in sugar beet arabinan to fermentable monosugars. Besides the main product arabinobiose, unknown arabinose oligomers are produced from highly branched arabinan when endoarabinanase was combined with exoarabinanase and/or arabinofuranosidase. All described arabinose releasing enzymes are temperature stable up to 50 degrees C and have a broad pH stability. This makes C1 arabinohydrolases suitable for many biotechnical applications, like co-fermentation bioethanol production.
S Kühnel; S W A Hinz; L Pouvreau; J Wery; H A Schols; H Gruppen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-20
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-07-13     Completed Date:  2010-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  8300-7     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands.
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MeSH Terms
Arabinose / metabolism
Beta vulgaris / metabolism*
Chromatography, Gel
Chromatography, Ion Exchange
Chrysosporium / enzymology*
Glycoside Hydrolases / metabolism*
Hydrogen-Ion Concentration
Molecular Weight
Polysaccharides / chemistry,  metabolism*
Substrate Specificity
Reg. No./Substance:
0/Polysaccharides; 11078-27-6/araban; 147-81-9/Arabinose; EC 3.2.1.-/Glycoside Hydrolases

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