| Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases. | |
MedLine Citation:
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PMID: 17982124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Remodeling of the dermal extracellular matrix occurs during photoaging. Here, the effect of repetitive UVB irradiation on dermal hyaluronic acid (HA) was examined. C57/BL6 mice were chronically (182 days) irradiated with UVB, and consecutive skin biopsies were collected during the irradiation period and afterward (300 and 400 days of age). UVB caused marked loss of HA from the papillary dermis and down-regulation of HA synthase 1 (HAS1), HAS2, and HAS3 mRNA expression. In contrast, hyaluronidases (HYAL) 1, HYAL2, and HA receptor CD44 were unchanged. Furthermore, transforming growth factor beta-1 (TGF-beta1) and TGF-beta1-receptor II expression were decreased in UVB-irradiated biopsies, and TGF-beta1 strongly induced HAS1 and HAS2 expression in cultured dermal fibroblasts. Therefore, TGF-beta1 might be one factor involved in UVB-induced down-regulation of HAS enzymes. In addition, total cell number and the percentage of proliferating fibroblasts in the papillary dermis of UVB-irradiated mice were decreased. Down-regulation of HAS2 by lentiviral overexpression of short hairpin RNA in vitro caused inhibition of HA synthesis, DNA synthesis, and migration of dermal fibroblasts. In conclusion, chronic UVB irradiation induces loss of HA from the dermis, thereby contributing to the quiescent phenotype of dermal fibroblasts. |
Authors:
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Guang Dai; Till Freudenberger; Petra Zipper; Ariane Melchior; Susanne Grether-Beck; Berit Rabausch; Jens de Groot; Sören Twarock; Helmut Hanenberg; Bernhard Homey; Jean Krutmann; Julia Reifenberger; Jens W Fischer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American journal of pathology Volume: 171 ISSN: 0002-9440 ISO Abbreviation: Am. J. Pathol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-11-05 Completed Date: 2008-01-25 Revised Date: 2013-06-06 |
Medline Journal Info:
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Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 1451-61 Citation Subset: AIM; IM |
Affiliation:
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Molekulare Pharmakologie, Institut für Pharmakologie and Klinische Pharmakologie, Universitätsklinkum Düsseldorf, Düsseldorf, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD44 / metabolism Cell Proliferation Cells, Cultured Chemotaxis Dermis / cytology, metabolism, radiation effects* Down-Regulation Fibroblasts / metabolism, physiology, radiation effects Glucuronosyltransferase / biosynthesis*, genetics Humans Hyaluronic Acid / metabolism* Hyaluronoglucosaminidase / metabolism Isoenzymes / biosynthesis Mice Mice, Inbred C57BL Receptors, Transforming Growth Factor beta / metabolism Transforming Growth Factor beta1 / metabolism Ultraviolet Rays / adverse effects* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD44; 0/Cd44 protein, mouse; 0/Isoenzymes; 0/Receptors, Transforming Growth Factor beta; 0/Transforming Growth Factor beta1; 9004-61-9/Hyaluronic Acid; EC 2.4.1.-/Has2 protein, mouse; EC 2.4.1.-/Has3 protein, mouse; EC 2.4.1.17/Glucuronosyltransferase; EC 2.4.1.212/hyaluronan synthase; EC 3.2.1.35/Hyaluronoglucosaminidase |
| Comments/Corrections | |
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