Document Detail


Chronic treadmill exercise in rats delicately alters the Purkinje cell structure to improve motor performance and toxin resistance in the cerebellum.
MedLine Citation:
PMID:  22837167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although exercise usually improves motor performance, the underlying cellular changes in the cerebellum remain to be elucidated. This study aimed to investigate whether and how chronic treadmill exercise in young rats induced Purkinje cell changes to improve motor performance and rendered the cerebellum less vulnerable to toxin insults. After 1-wk familiarization of treadmill running, 6-wk-old male Wistar rats were divided into exercise and sedentary groups. The exercise group was then subjected to 8 wk of exercise training at moderate intensity. The rotarod test was carried out to evaluate motor performance. Purkinje cells in cerebellar slices were visualized by lucifer yellow labeling in single neurons and by calbindin immunostaining in groups of neurons. Compared with sedentary control rats, exercised rats not only performed better in the rotarod task, but also showed finer Purkinje cell structure (higher dendritic volume and spine density with the same dendritic field). The exercise-improved cerebellar functions were further evaluated by monitoring the long-lasting effects of intraventricular application of OX7-saporin. In the sedentary group, OX7-saporin treatment retarded the rotarod performance and induced ∼60% Purkinje cell loss in 3 wk. As a comparison, the exercise group showed much milder injuries in the cerebellum by the same toxin treatment. In conclusion, exercise training in young rats increased the dendritic density of Purkinje cells, which might play an important role in improving the motor performance. Furthermore, as Purkinje cells in the exercise group were relatively toxin resistant, the exercised rats showed good motor performance, even under toxin-treated conditions.
Authors:
Tung-Yi Huang; Lung-Sheng Lin; Keng-Chi Cho; Shean-Jen Chen; Yu-Min Kuo; Lung Yu; Fong-Sen Wu; Jih-Ing Chuang; Hsiun-Ing Chen; Chauying J Jen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-26
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  113     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-17     Completed Date:  2013-02-05     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  889-95     Citation Subset:  IM    
Affiliation:
Department of Physiology, National Cheng Kung University, Tainan, Taiwan, Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / administration & dosage,  toxicity*
Biological Markers / metabolism
Calcium-Binding Protein, Vitamin D-Dependent / metabolism
Cell Shape
Fluorescent Antibody Technique
Immunoconjugates / administration & dosage,  toxicity*
Immunotoxins / administration & dosage,  toxicity*
Injections, Intraventricular
Male
Microscopy, Fluorescence
Motor Activity* / drug effects
Neurotoxicity Syndromes / etiology,  metabolism,  pathology,  prevention & control*
Physical Exertion*
Purkinje Cells / drug effects*,  metabolism,  pathology
Rats
Rats, Wistar
Ribosome Inactivating Proteins, Type 1 / administration & dosage,  toxicity*
Running
Sedentary Lifestyle
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Biological Markers; 0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Immunoconjugates; 0/Immunotoxins; 0/OX7-saporin; 0/Ribosome Inactivating Proteins, Type 1; 0/calbindin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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