Document Detail

Chronic stimulation-induced changes of myosin light chains at the mRNA and protein levels in rat fast-twitch muscle.
MedLine Citation:
PMID:  2465148     Owner:  NLM     Status:  MEDLINE    
Transitions in the expression of the myosin light chains (LC) were investigated in fast-twitch muscles of the rat during chronic (10 h/day), low-frequency (10 Hz) stimulation. Changes were followed at the mRNA level by Northern blot analysis and in vitro translation, as well as at the protein level by electrophoresis under denaturing and nondenaturing conditions. In vivo synthesis of the light chains was assessed by measuring the incorporation of intramuscularly injected [35S]methionine. Chronic stimulation induced a transition in the isomyosin pattern with an increase of FM3, a concomitant decrease in FM1 and, after longer stimulation periods, the appearance of low concentrations of the slow isomyosin. These changes were accompanied by an elevated LC1f/LC3f ratio and increases in the amounts of both the LC1sb and, to a lesser degree, LC2s proteins. Alterations in the amounts of specific mRNAs were the same whether determined by Northern blot analysis or by in vitro translation of total RNA preparations from the same muscles. Generally, the changes in the relative concentrations of fast and slow light-chain proteins agreed with the changes detected at the mRNA level and the alterations in protein synthesis detected with the use of an in vivo labeling assay. An exception was the elevated tissue content of LC2s where no changes were detectable in the concentration of its mRNA as determined by in vitro translation or in vivo synthesis. The increase in LC2s protein may, therefore, have been due to reduced degradation. In addition, the decrease in LC3f was more pronounced at the protein level than at the mRNA level. This might indicate an increased turnover of LC3f or the existence of additional post-transcriptional regulations of LC3f expression.
B J Kirschbaum; J A Simoneau; A Bär; P J Barton; M E Buckingham; D Pette
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  179     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-04-04     Completed Date:  1989-04-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  23-9     Citation Subset:  IM    
Fakultät für Biologie, Universität Konstanz, Federal Republic of Germany.
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MeSH Terms
Blotting, Northern
Electric Stimulation
Electrophoresis / methods
Methionine / metabolism
Muscle Contraction*
Muscles / innervation,  metabolism*,  physiology
Myosin Subfragments
Myosins / analysis*,  genetics
Peptide Fragments / analysis
Poly A / analysis
Protein Biosynthesis
RNA / analysis
RNA, Messenger / analysis*,  isolation & purification
Rats, Inbred Strains
Reg. No./Substance:
0/Myosin Subfragments; 0/Peptide Fragments; 0/RNA, Messenger; 24937-83-5/Poly A; 63-68-3/Methionine; 63231-63-0/RNA; EC

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