| Chronic reduction in cardiac output induces hypoxic signaling in larval zebrafish even at a time when convective oxygen transport is not required. | |
| | |
MedLine Citation:
|
PMID: 20571107 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In the present study, the zebrafish breakdance mutant (bre) was used to assess the role of blood flow in development because it has been previously shown that bre larvae have a chronically reduced cardiac output as a result of ventricular contraction following only every second atrial contraction in addition to an atrial bradycardia. We confirmed a 50% reduction compared with control fish and further showed that blood flow in the caudal part of the dorsal aorta decreased by 80%. Associated with these reductions in blood flow were indications of developmental retardation in bre mutants, specifically delayed hatching, reduced cell proliferation, and a transiently decreased growth rate. Surprisingly, an increased red blood cell concentration and an earlier appearance of trunk vessels in bre larvae indicated some compensation to convective oxygen transport, although in previous studies it has been shown that zebrafish larvae at this stage obtain oxygen by bulk diffusion. In bre animals immunohistochemical analyses showed a significant increase in hypoxia inducible factor 1 (HIF)-α protein expression, comparable with wild-type larvae that were raised under hypoxic conditions. Accordingly, the expression of some hif downstream genes was affected. Furthermore, Affymetrix microarray analyses revealed a large number of genes that were differently expressed comparing control and bre larvae, and the number even increased with proceeding development. The results showed that a chronic reduction in blood flow generated hypoxic molecular signals despite partial compensation by increased oxygen carrying capacity and transiently slowed the overall development of zebrafish bre larvae. |
| | |
Authors:
|
Renate Kopp; Thorsten Schwerte; Margit Egg; Adolf Michael Sandbichler; Bernhard Egger; Bernd Pelster |
Related Documents
:
|
1096397 - Cardiac output determinations with ear piece densitometry. 9789577 - Role of nitric oxide in the regulation of regional blood flow and metabolism in anaesth... 2044577 - Extravascular chest wall technetium 99m diethylene triamine penta-acetic acid: implicat... 2901217 - Regional blood flow responses to vasodilators and inotropes in congestive heart failure. 2174037 - A novel antioxidant role for hemoglobin. the comproportionation of ferrylhemoglobin wit... 8311587 - Fetal cardiac bypass: improved placental function with moderately high flow rates. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-22 |
Journal Detail:
|
Title: Physiological genomics Volume: 42A ISSN: 1531-2267 ISO Abbreviation: Physiol. Genomics Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-09-20 Completed Date: 2010-12-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9815683 Medline TA: Physiol Genomics Country: United States |
Other Details:
|
Languages: eng Pagination: 8-23 Citation Subset: IM |
Affiliation:
|
Institute of Zoology and Center for Molecular Biosciences, University of Innsbruck, Innsbruck, Austria. Renate.Kopp@uibk.ac.at |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anoxia / genetics, metabolism* Biological Transport / genetics, physiology CLOCK Proteins / genetics Cardiac Output / genetics, physiology* Cell Cycle Proteins / genetics Cyclin B1 / genetics Erythropoietin / genetics Hypoxia-Inducible Factor 1, alpha Subunit / genetics, metabolism Immunohistochemistry Larva / genetics, metabolism, physiology* Oligonucleotide Array Sequence Analysis Oxygen / metabolism* Polymerase Chain Reaction Vascular Endothelial Growth Factor A / genetics, metabolism Zebrafish / genetics, metabolism, physiology* Zebrafish Proteins / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Cell Cycle Proteins; 0/Cyclin B1; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Vascular Endothelial Growth Factor A; 0/Zebrafish Proteins; 0/epo protein, zebrafish; 11096-26-7/Erythropoietin; 7782-44-7/Oxygen; EC 2.3.1.48/CLOCK Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hyperglycemia Management Using Insulin in the Acute Care Setting: Therapies and Strategies for Care ...
Next Document: Confirmation of multiple seizure susceptibility QTLs on chromosome 15 in C57BL/6J and DBA/2J inbred ...