| Chronic peripheral lymphatic cannulation in the dog. | |
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MedLine Citation:
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PMID: 8258988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Peripheral lymph collected acutely has been commonly sampled as representative of non-visceral interstitial fluid. By developing a prenodal lymphatic-lymphatic (L-L) shunt, we were able to collect peripheral lymph for 3-5 days in unanesthetized dogs. The L-L shunt was constructed entirely of medical grade silicone rubber tubing designed with a slip of coupling which allowed the shunt to be disconnected for lymph collection and reconnected at night. Average peripheral lymph flow (4.9 ml/hr leg) in unanesthetized dogs was almost twice the flow rate previously observed in anesthetized dogs. The average lymph/plasma total protein concentration ratio (0.16), however, was similar to that previously found in anesthetized dogs. Lymph protein concentration fell with the collection during the day and became more concentrated at night. Lymph flow did not change greatly during daytime collection. Average peripheral lymph collection volume was greater than 200 ml/dog. The L-L shunt allows collection of prenodal-lymph in experiments where unanesthetized dogs are required (e.g., feeding studies). They also are useful when multiple protocols are conducted on the same dog or when large volumes of peripheral lymph are required. |
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Authors:
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C H Sloop; M Lefevre; C K Castle; L Wong |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Lymphology Volume: 26 ISSN: 0024-7766 ISO Abbreviation: Lymphology Publication Date: 1993 Sep |
Date Detail:
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Created Date: 1994-01-19 Completed Date: 1994-01-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155112 Medline TA: Lymphology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 143-9 Citation Subset: IM |
Affiliation:
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Division of Lipoprotein Metabolism and Pathophysiology, Louisiana State University Medical Center, Baton Rouge. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Proteins / analysis Catheterization / methods* Dogs Lymph* / chemistry, physiology |
| Grant Support | |
ID/Acronym/Agency:
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HL-25596/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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