Document Detail


Chronic nicotine exposure systemically alters microRNA expression profiles during post-embryonic stages in Caenorhabditis elegans.
MedLine Citation:
PMID:  23765240     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tobacco smoking is associated with many diseases. Addiction is of the most notorious tobacco-related syndrome and is mainly attributed to nicotine. In this study, we employed Caenorhabditis elegans as a biological model to systemically investigate the effect of chronic nicotine exposure on microRNA (miRNA) expression profile and their regulated biochemical pathways. Nicotine treatment (20 µM and 20 mM) was limited to the post-embryonic stage from L1 to L4 (∼31 h) period after which worms were collected for genome-wide miRNA profiling. Our results show that nicotine significantly altered the expression patterns of 40 miRNAs. The effect was proportional to the nicotine dose and was expected to have an additive, more robust response. Based on pathway enrichment analyses coupled with nicotine-induced miRNA patterns, we inferred that miRNAs as a system mediates "regulatory hormesis", manifested in biphasic behavioral and physiological phenotypes. We proposed a model where nicotine addiction is mediated by miRNAs' regulation of fos-1 and is maintained by epigenetic factors. Thus, our study offers new insights for a better understanding of the sensitivity of early developmental stages to nicotine.
Authors:
Faten A Taki; Xiaoping Pan; Baohong Zhang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  229     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2013-09-30     Completed Date:  2013-12-09     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  79-89     Citation Subset:  IM    
Copyright Information:
© 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / drug effects*,  growth & development
Epigenesis, Genetic*
Gene Expression Regulation, Developmental / drug effects*
Humans
MicroRNAs / drug effects
Nicotine / toxicity*
RNA, Messenger / genetics
Transcriptome
Grant Support
ID/Acronym/Agency:
R03 DA032515/DA/NIDA NIH HHS; R03DA032515/DA/NIDA NIH HHS
Chemical
Reg. No./Substance:
0/MicroRNAs; 0/RNA, Messenger; 54-11-5/Nicotine
Comments/Corrections

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