| Chronic morphine treatment up-regulates mu opioid receptor binding in cells lacking filamin A. | |
| | |
MedLine Citation:
|
PMID: 17897634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We investigated the effects of morphine and other agonists on the human mu opioid receptor (MOP) expressed in M2 melanoma cells, lacking the actin cytoskeleton protein filamin A and in A7, a subclone of the M2 melanoma cells, stably transfected with filamin A cDNA. The results of binding experiments showed that after chronic morphine treatment (24 h) of A7 cells, MOP-binding sites were down-regulated to 63% of control, whereas, unexpectedly, in M2 cells, MOP binding was up-regulated to 188% of control naive cells. Similar up-regulation was observed with the agonists methadone and levorphanol. The presence of antagonists (naloxone or CTAP) during chronic morphine treatment inhibited MOP down-regulation in A7 cells. In contrast, morphine-induced up-regulation of MOP in M2 cells was further increased by these antagonists. Chronic morphine desensitized MOP in A7 cells, i.e., it decreased DAMGO-induced stimulation of GTPgammaS binding. In M2 cells DAMGO stimulation of GTPgammaS binding was significantly greater than in A7 cells and was not desensitized by chronic morphine. Pertussis toxin treatment abolished morphine-induced receptor up-regulation in M2 cells, whereas it had no effect on morphine-induced down-regulation in A7 cells. These results indicate that, in the absence of filamin A, chronic treatment with morphine, methadone or levorphanol leads to up-regulation of MOP, to our knowledge, the first instance of opioid receptor up-regulation by agonists in cell culture. |
| | |
Authors:
|
Irma Onoprishvili; Eric J Simon |
Related Documents
:
|
8737244 - Prosaposin and prosaptide, a peptide from prosaposin, induce an increase in ganglioside... 2067534 - Dominant lethal cell mutants detected by the autoradiographic assay for exotoxin a resi... 2047654 - Shiga toxin: intestinal cell receptors and pathophysiology of enterotoxic effects. 5663574 - Response of cultured mammalian cells to diphtheria toxin. 3. inhibition of protein synt... 23186684 - Purification of fructooligosaccharides by immobilized yeast cells and identification of... 19164894 - Cathepsin l mediates resveratrol-induced autophagy and apoptotic cell death in cervical... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-08-16 |
Journal Detail:
|
Title: Brain research Volume: 1177 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-10-19 Completed Date: 2008-01-15 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 9-18 Citation Subset: IM |
Affiliation:
|
Department of Psychiatry, New York University School of Medicine, New York, NY 10016, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Western Cell Line Cell Line, Tumor Contractile Proteins / deficiency, physiology* Data Interpretation, Statistical Diprenorphine / pharmacology Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / pharmacology Guanosine 5'-O-(3-Thiotriphosphate) / metabolism Humans Ligands Melanoma / genetics, pathology Microfilament Proteins / deficiency, physiology* Morphine / pharmacology* Narcotic Antagonists / metabolism, pharmacology Narcotics / pharmacology* Pertussis Toxin / pharmacology Radioligand Assay Receptors, Opioid, mu / agonists, drug effects*, metabolism Tubulin / pharmacology Up-Regulation / drug effects |
| Grant Support | |
ID/Acronym/Agency:
|
DA-072545/DA/NIDA NIH HHS; DA00017/DA/NIDA NIH HHS; R01 DA000017-40S2/DA/NIDA NIH HHS; T32 DA007254-15/DA/NIDA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Contractile Proteins; 0/Ligands; 0/Microfilament Proteins; 0/Narcotic Antagonists; 0/Narcotics; 0/Receptors, Opioid, mu; 0/Tubulin; 0/filamins; 100929-53-1/Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; 14357-78-9/Diprenorphine; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 57-27-2/Morphine; EC 2.4.2.31/Pertussis Toxin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Angiotensin-converting enzyme 2 and new insights into the renin-angiotensin system.
Next Document: A novel C-terminal truncation SCN5A mutation from a patient with sick sinus syndrome, conduction dis...