| Chronic low frequency stimulation reduces myosin phosphorylation in rabbit fast twitch muscle. | |
MedLine Citation:
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PMID: 1330259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of 1-12 days of electrical stimulation (10 Hz) on the ability to phosphorylate the P-light chain of myosin was studied in rabbit tibialis anterior muscle. Myosin phosphorylation was induced by exposure of the stimulated muscle and that of the contralateral leg to a single conditioning stimulus train (5 Hz) for 25 s via the motor nerve. Isometric tension was measured as were the myosin light chain composition and the activities of the enzymes responsible for phosphorylation and dephosphorylation. A computer simulation of the potential effect of a stimulation-induced disruption of Ca2+ metabolism on phosphorylation was also performed. Chronic stimulation for as little as 1 day eliminated light chain phosphorylation and reduced the myosin light chain kinase activity by approximately 36%. Conversely, phosphatase activity and light chain composition were unaffected. The model demonstrated that a slight depression in the magnitude of the Ca2+ transient could potentially attenuate phosphorylation. The data suggest that phosphorylation of myosin is extremely sensitive to prolonged muscle activity. Furthermore, it appears more likely that this sensitivity is related to regulation of intracellular free Ca2+ than to the other elements of the calmodulin-dependent system for myosin phosphorylation examined. |
Authors:
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G A Klug; M Biedermann; M E Houston; D Stuart; M Mumby; J T Stull |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Canadian journal of physiology and pharmacology Volume: 70 ISSN: 0008-4212 ISO Abbreviation: Can. J. Physiol. Pharmacol. Publication Date: 1992 Jun |
Date Detail:
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Created Date: 1992-12-23 Completed Date: 1992-12-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372712 Medline TA: Can J Physiol Pharmacol Country: CANADA |
Other Details:
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Languages: eng Pagination: 859-65 Citation Subset: IM |
Affiliation:
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Department of Exercise and Movement Science, University of Oregon, Eugene 97403. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Electric Stimulation Isometric Contraction / physiology* Male Muscle Tonus / physiology Muscles / enzymology, metabolism*, physiology Myosin-Light-Chain Kinase / metabolism Myosin-Light-Chain Phosphatase Myosins / metabolism*, physiology Phosphoprotein Phosphatases / metabolism Phosphorylation Rabbits Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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AR 39583/AR/NIAMS NIH HHS; HL 06296/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7440-70-2/Calcium; EC 2.7.11.18/Myosin-Light-Chain Kinase; EC 3.1.3.16/Phosphoprotein Phosphatases; EC 3.1.3.53/Myosin-Light-Chain Phosphatase; EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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