Document Detail


Chronic hypoxia delays myocardial lactate dehydrogenase maturation in young rats.
MedLine Citation:
PMID:  12719765     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of exposure to hypobaric hypoxia for 4 weeks (oxygen pressure = 106 hPa), equivalent to 5500 m in altitude) on myocardial total lactate dehydrogenase (tLDH) activity and isoform (H and M) composition was comparatively studied in growing (4.5 weeks old) and in adult (4.5 months old) male rats. The consequences of the hypoxia-induced anorexia were checked in growing rats using a pair-fed group. Exposure to hypoxia induced a significant decrease in the H/tLDH ratio in the left (LV) and right ventricle (RV) of growing and adult rats. In adult rats this alteration was mainly a consequence of the significant increase in the specific activity of the M isomer, which resulted in an increase in the overall LDH activity. In contrast, in the LV of young rats exposed to hypoxia, the specific activity of the M isomer was similar to that of normoxic animals while the H isomer activity was significantly lower than in normoxic rats, and the overall LDH activity remained unchanged. These effects were specifically due to hypoxia per se since no significant alterations were observed in pair-fed animals. In the hypertrophied RV, the alteration of H and M isomers following hypoxia was similar to that observed in adults (i.e. no change in H and an increase in M isoform). We conclude that the well-known hypoxia-induced decrease in the H/tLDH ratio is governed by different age-dependent mechanisms. In adult rats, hypoxia may induce in both ventricles a stimulating effect on M isomer expression. In the LV of growing rats this stress could inhibit the H isomer maturation without any effect on the M isomer. In the RV of growing rats this effect could have been counteracted by the growth effect of the hypertrophying process.
Authors:
Z Daneshrad; M Verdys; O Birot; F Troff; A X Bigard; A Rossi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental physiology     Volume:  88     ISSN:  0958-0670     ISO Abbreviation:  Exp. Physiol.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-29     Completed Date:  2004-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9002940     Medline TA:  Exp Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  405-13     Citation Subset:  IM    
Affiliation:
Laboratoire de Bioénergétique, Université Joseph Fourier, Grenoble, France.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Anoxia / metabolism*
Body Weight / physiology
Cardiomegaly / metabolism,  pathology
Chronic Disease
Energy Intake / physiology
Energy Metabolism / physiology
Heart Ventricles / enzymology,  pathology
Isoenzymes / metabolism*
L-Lactate Dehydrogenase / metabolism*
Male
Myocardium / enzymology*,  pathology
Rats
Rats, Wistar
Substrate Specificity
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 1.1.1.27/L-Lactate Dehydrogenase

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