Document Detail


Chronic food restriction and acute food deprivation decrease mRNA levels of opioid peptides in arcuate nucleus.
MedLine Citation:
PMID:  8928900     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although opioid administration induces food intake, the relationship between endogenous opioid synthesis and food consumption is unclear. Two studies examined the effects of food restriction and deprivation on opioid mRNA levels in the arcuate nucleus (ARC) of the rat. Body weight significantly decreased following food restriction and deprivation (P < 0.0001). In experiment 1, food restriction of 10,20,30, and 40% (g) of ad libitum intake for 14 days decreased proDynorphin (proDyn), proEnkephalin (proEnk), and proOpiomelanocortin (POMC) mRNA levels in a linear fashion relative to changes in body weight (r = 0.398, P = 0.0011; r = 0.455, P = 0.0028; r = 0.292, P = 0.0642, respectively). In experiment 2, 48 h deprivation significantly decreased mRNA levels of proDyn and POMC by 23.7% (P < 0.05) and 45.6% (P < 0.01), respectively, whereas 24 h food deprivation decreased POMC mRNA by 43.% (P < 0.01). proEnk mRNA was not affected by 24- or 48-h food deprivation. Restricting food intake suppressed mRNA levels of proDyn, proEnk, and POMC by 29.7, 22.3, and 44.4%, respectively, in 20% restricted rats and by 35.5, 26.8, and 45.6%, respectively, in 40%restricted rats (P < 0.01). It appears that ARC mRNA levels of proDyn, proEnk, and POMC are directly related to the amount of food consumed and/or changes in body weight in food-restricted and food-deprived rats.
Authors:
E M Kim; C C Welch; M K Grace; C J Billington; A S Levine
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  270     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-11-27     Completed Date:  1996-11-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R1019-24     Citation Subset:  IM    
Affiliation:
Research and Medicine Service. Department of Veterans Affairs Medical Center, Minneapolis, Minnesota, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arcuate Nucleus / metabolism*
Body Weight
Enkephalins / genetics,  metabolism*
Food Deprivation / physiology*
Male
Pro-Opiomelanocortin / genetics,  metabolism*
Protein Precursors / genetics,  metabolism*
RNA, Messenger / metabolism*
Rats
Rats, Sprague-Dawley
Time Factors
Grant Support
ID/Acronym/Agency:
DA-03999/DA/NIDA NIH HHS; DA-07097/DA/NIDA NIH HHS; DK-42698/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Enkephalins; 0/Protein Precursors; 0/RNA, Messenger; 0/proenkephalin; 66796-54-1/Pro-Opiomelanocortin; 93443-35-7/preproenkephalin

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