| Chronic exposure to inorganic lead increases high-threshold voltage-gated calcium currents in rat pheochromocytoma (PC12) cells. | |
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MedLine Citation:
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PMID: 9406956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat pheochromocytoma (PC12) cells were exposed to lead acetate (0, 10, 25 and 50 microM) in their growth media for up to 12 weeks. High-threshold voltage-gated calcium currents were recorded each week from nerve growth factor-differentiated PC12 cells using the whole-cell patch-clamp technique. Chronic exposure for 1 month did not modify peak or sustained calcium current amplitudes in lead-treated cells when compared to sister control cultures. Two month exposure to 25 and 50 microM significantly increased peak and sustained calcium current amplitudes, while 10 microM had little effect. During the third month of exposure, peak and sustained calcium current amplitudes remained increased in the cells exposed to 25 and 50 microM lead acetate. By the end of the second month of exposure to 25 and 50 microM lead acetate, the voltage at which maximal current amplitude was attained shifted from + 10 mV to 0 mV. The observed effects of toxicologically relevant lead concentrations on high-threshold calcium currents in chronically exposed mammalian cells provide further support for the notion that at least one cellular target of the heavy metal's neurotoxic action may be the voltage-gated calcium channel. |
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Authors:
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C C Hegg; V Miletic |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 772 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1998-02-19 Completed Date: 1998-02-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 63-70 Citation Subset: IM; S |
Affiliation:
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Department of Comparative Biosciences and the Environmental Toxicology Center, University of Wisconsin, Madison 53706-1102, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium Channels / drug effects* Electric Conductivity Ion Channel Gating* Lead / toxicity* Membrane Potentials / drug effects PC12 Cells Patch-Clamp Techniques Rats Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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NS 34870/NS/NINDS NIH HHS; T32 ES07015/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channels; 7439-92-1/Lead |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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