Document Detail


Chronic exposure of human macrophages in vitro to morphine and methadone induces a putative tolerant/dependent state.
MedLine Citation:
PMID:  18396338     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have shown previously that whereas acute exposure of cultured murine peritoneal macrophages inhibits phagocytosis, chronic exposure results in a putative tolerant/dependent state. We now report similar observations using human cultured monocyte-derived macrophages (hMDM) from a control population and from methadone patients. With hMDM, acute exposure to morphine and methadone inhibited phagocytosis in a dose-dependent manner. In contrast, chronic exposure resulted in eventual normalization of phagocytosis, indicating that a putative tolerant state to the opiates had developed. When opiates were withdrawn from chronically-exposed, tolerized hMDM, phagocytosis was once again depressed. The duration of withdrawal-induced depression lasted several hours, which is much longer than evidenced previously with murine macrophages. These data identify well with various in vivo studies on immune effects of opiate withdrawal; and, in so-doing, supplement ongoing speculation that opiate withdrawal is likely to have serious impact on host defenses of street heroin addicts.
Authors:
Manuel Delgado-Vélez; Ana Lugo-Chinchilla; Luis Lizardo; Irmarie Morales; Yarelis Robles; Nimaris Bruno; José W Rodríguez; Eddy Ríos-Olivares; Miguel Correa; Fernando L Renaud
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-04-08
Journal Detail:
Title:  Journal of neuroimmunology     Volume:  196     ISSN:  0165-5728     ISO Abbreviation:  J. Neuroimmunol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-06-02     Completed Date:  2008-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8109498     Medline TA:  J Neuroimmunol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  94-100     Citation Subset:  IM    
Affiliation:
Biology Department, University of Puerto Rico, Río Piedras Campus, POB 23360, San Juan, Puerto Rico 00931-3360.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Analysis of Variance
Cell Differentiation / drug effects,  physiology
Cells, Cultured
Dose-Response Relationship, Drug
Drug Tolerance / physiology*
Female
Humans
Macrophages / drug effects*
Male
Methadone / pharmacology*
Morphine / pharmacology*
Naloxone / pharmacology
Narcotic Antagonists / pharmacology
Narcotics / pharmacology*
Substance Withdrawal Syndrome / etiology
Time Factors
Grant Support
ID/Acronym/Agency:
5R25GM061151/GM/NIGMS NIH HHS; EHR-0314557//PHS HHS; G12RR03035/RR/NCRR NIH HHS; P20 RR-016470/RR/NCRR NIH HHS; SO6GM-08102/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Narcotic Antagonists; 0/Narcotics; 465-65-6/Naloxone; 57-27-2/Morphine; 76-99-3/Methadone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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