Document Detail

Chronic exposure of cultured transformed mouse epidermal cells to transforming growth factor-beta 1 induces an epithelial-mesenchymal transdifferentiation and a spindle tumoral phenotype.
MedLine Citation:
PMID:  8547217     Owner:  NLM     Status:  MEDLINE    
Transformed mouse epidermal keratinocytes of the cell line PDV, when cultured under the presence of transforming growth factor-beta 1 (TGF-beta 1), escaped the block of growth exerted by this factor in normal keratinocytes and underwent marked changes in cell differentiation. TGF-beta 1 induced disruption of epithelial interactions, dispersion of cells, increased local movement, and conversion to a fibroblast-like morphology. These changes were reversible and correlated with down-regulation of epithelial protein markers such as E-cadherin and cytokeratins and upregulation of vimentin. TGF-beta 1-treated cells with a fibroblast-like phenotype induced spindle cell carcinomas upon transplantation in athymic nude mice, whereas untreated PDV cells or fusiform cells reverted to the epithelial phenotype and produced well-differentiated squamous cell carcinomas. Nontumorigenic immortalized epidermal keratinocytes, when grown under the presence of TGF-beta 1, did not transdifferentiate to a mesenchymal phenotype, their proliferation was blocked, and cells finally died. These results suggest a role of TGF-beta 1 in the progression of squamous carcinoma cells to spindle carcinomas in mouse skin carcinogenesis.
C Caulín; F G Scholl; P Frontelo; C Gamallo; M Quintanilla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  6     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1996-02-20     Completed Date:  1996-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1027-35     Citation Subset:  IM    
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Cientificas-Universidad Autonoma de Madrid, Spain.
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MeSH Terms
Biological Markers / chemistry
Carcinoma, Squamous Cell / chemically induced*,  genetics
Cell Differentiation / drug effects
Cell Line, Transformed
Chronic Disease
Epithelial Cells
Epithelium / drug effects
Keratinocytes / cytology,  drug effects*
Mesoderm / cytology,  drug effects*
Mice, Nude
Transforming Growth Factor beta / pharmacology*
Reg. No./Substance:
0/Biological Markers; 0/Transforming Growth Factor beta

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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