Document Detail

Chronic exercise in dogs increases coronary vascular nitric oxide production and endothelial cell nitric oxide synthase gene expression.
MedLine Citation:
PMID:  7507417     Owner:  NLM     Status:  MEDLINE    
Recently, we have shown that chronic exercise increases endothelium-derived relaxing factor (EDRF)/nitric oxide (NO)-mediated epicardial coronary artery dilation in response to brief occlusion and acetylcholine. This finding suggests that exercise can provide a stimulus for the enhanced production of EDRF/NO, thus possibly contributing to the beneficial effects of exercise on the cardiovascular system. Therefore, the purpose of the present study was to examine whether chronic exercise could influence the production of NO (measured as the stable degradation product, nitrite) and endothelial cell NO synthase (ECNOS) gene expression in vessels from dogs after chronic exercise. To this end, dogs were exercised by running on a treadmill (9.5 km/h for 1 hour, twice daily) for 10 days, and nitrite production in large coronary vessels and microvessels and ECNOS gene expression in aortic endothelial extracts were assessed. Acetylcholine (10(-7) to 10(-5) mol/L) dose-dependently increased the release of nitrite (inhibited by nitro-L-arginine) from coronary arteries and microvessels in control and exercised dogs. Moreover, acetylcholine-stimulated nitrite production was markedly enhanced in large coronary arteries and microvessels prepared from hearts of dogs after chronic exercise compared with hearts from control dogs. One potential mechanism that may contribute to the enhanced production of nitrite in vessels from exercised dogs may be the induction of the calcium-dependent ECNOS gene. Steady-state mRNA levels for ECNOS were significantly higher than mRNA levels for von Willebrand's factor (vWF, a specific endothelial cell marker) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, a constitutively expressed gene) in exercised dogs.(ABSTRACT TRUNCATED AT 250 WORDS)
W C Sessa; K Pritchard; N Seyedi; J Wang; T H Hintze
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation research     Volume:  74     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-02-28     Completed Date:  1994-02-28     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  349-53     Citation Subset:  IM    
Department of Physiology, New York Medical College, Valhalla.
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MeSH Terms
Amino Acid Oxidoreductases / genetics*
Coronary Vessels / metabolism*
Endothelium, Vascular / cytology,  enzymology*
Gene Expression / physiology*
Nitric Oxide / biosynthesis*
Nitric Oxide Synthase
Nitrites / metabolism
Physical Exertion*
RNA / metabolism
Time Factors
Grant Support
Reg. No./Substance:
0/Nitrites; 10102-43-9/Nitric Oxide; 63231-63-0/RNA; EC Oxide Synthase; EC 1.4.-/Amino Acid Oxidoreductases

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