| Chronic ethanol downregulates Gal-beta-1,4GlcNAc alpha 2,6-sialyltransferase and Gal-beta-1,3GlcNAc alpha 2,3-sialyltransferase mRNAs in rat liver. | |
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MedLine Citation:
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PMID: 9113274 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously demonstrated that chronic ethanol specifically decreases the hepatic level and rate of synthesis of 2,6-sialyltransferase (2,6-ST). To understand its mechanism of action, effects of 8 weeks of chronic ethanol feeding on the expression of sialyltransferase (ST) genes in rat liver and kidneys were determined by Northern-blot analysis of ST mRNAs. It was found that, compared with the pair-fed control rats, the percentage decreases in ST mRNAs in the ethanol-fed group were as follows: liver-Gal-beta-1,4GlcNAc alpha 2,6-ST (2,6-ST): 59% (p < 0.001); liver-Gal-beta-1,3GlcNAc alpha 2,3-ST (2,3-ST): 32% (p < 0.01); and kidneys-2,6-ST: 5% (NS). In contrast, glyceral-dehyde-3-phosphate dehydrogenase mRNA in both liver and kidneys was unaffected by the same ethanol treatment. Taken together, these results demonstrate that chronic ethanol downregulates the expression of 2,6-ST and 2,3-ST genes in rat liver. |
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Authors:
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M N Rao; M R Lakshman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Alcoholism, clinical and experimental research Volume: 21 ISSN: 0145-6008 ISO Abbreviation: Alcohol. Clin. Exp. Res. Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-07-22 Completed Date: 1997-07-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7707242 Medline TA: Alcohol Clin Exp Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 348-51 Citation Subset: IM |
Affiliation:
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Department of Medicine, George Washington University, Washington, DC, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alcoholism
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enzymology*,
genetics Animals Down-Regulation / drug effects, genetics Liver / drug effects*, enzymology Male RNA, Messenger / genetics* Rats Rats, Wistar Sialyltransferases / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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AA08149/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase; EC 2.4.99.4/beta-galactoside alpha-2,3-sialyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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