Document Detail


Chronic cystamine treatment inhibits small artery remodelling in rats.
MedLine Citation:
PMID:  17657163     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: We investigated whether the tissue transglutaminase inhibitor cystamine is able to inhibit remodelling of small arteries in vivo, a possibility suggested by recent in vitro experiments. METHODS: Using osmotic minipumps, phenylephrine, cystamine and/or amlodipine were infused for 1-2 weeks into 9-week-old Wistar rats. Small arteries were then removed for pressure myograph investigation. RESULTS: Phenylephrine infusion caused inward remodelling of the small arteries compared to vehicle infusion. The remodelling was abolished by concomitant infusion with cystamine; blood pressure was unaffected. Second, we investigated whether cystamine was able to inhibit outward remodelling. Rats were first infused with phenylephrine for 1 week, and some were infused for a further week with amlodipine with or without cystamine. Amlodipine caused 24% outward remodelling compared to vessels from rats at completion of the phenylephrine infusion. The outward remodelling was attenuated 86% by concomitant cystamine infusion. A series of in vitro experiments supported the inhibitory action of cystamine on tissue transglutaminase. CONCLUSION: The ability of cystamine to inhibit inward remodelling independent of blood pressure is consistent with a role of tissue transgluaminase in this process. It remains to be determined if the ability of cystamine to inhibit outward remodelling also involves inhibition of tissue transglutaminase.
Authors:
Ashkan Eftekhari; Awahan Rahman; Louise Holm Schaebel; Hua Chen; Claus Vitrup Rasmussen; Christian Aalkjaer; Carsten Leander Buus; Michael J Mulvany
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-26
Journal Detail:
Title:  Journal of vascular research     Volume:  44     ISSN:  1423-0135     ISO Abbreviation:  J. Vasc. Res.     Publication Date:  2007  
Date Detail:
Created Date:  2007-10-24     Completed Date:  2007-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  471-82     Citation Subset:  IM    
Copyright Information:
Copyright 2007 S. Karger AG, Basel.
Affiliation:
Department of Pharmacology, University of Aarhus, Aarhus, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Amlodipine / administration & dosage,  pharmacology*,  therapeutic use
Animals
Antihypertensive Agents / administration & dosage,  pharmacology*,  therapeutic use
Blood Pressure / drug effects
Collagen / metabolism
Cystamine / administration & dosage,  pharmacology*,  therapeutic use
Disease Models, Animal
Dose-Response Relationship, Drug
Enzyme Inhibitors / administration & dosage,  pharmacology*,  therapeutic use
GTP-Binding Proteins / antagonists & inhibitors*,  metabolism
Gels
Heart Rate / drug effects
Hypertension / drug therapy*,  enzymology,  pathology,  physiopathology
Immunohistochemistry
Infusion Pumps
Male
Mesenteric Arteries / drug effects*,  enzymology,  pathology
Microscopy, Confocal
Phenylephrine
Rats
Rats, Wistar
Transglutaminases / antagonists & inhibitors*,  metabolism
Vasoconstriction / drug effects
Vasoconstrictor Agents
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Enzyme Inhibitors; 0/Gels; 0/Vasoconstrictor Agents; 51-85-4/Cystamine; 59-42-7/Phenylephrine; 88150-42-9/Amlodipine; 9007-34-5/Collagen; EC 2.3.2.-/transglutaminase 2; EC 2.3.2.13/Transglutaminases; EC 3.6.1.-/GTP-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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