Document Detail

Chronic caffeine or theophylline intake during pregnancy inhibits A1 receptor function in the rat brain.
MedLine Citation:
PMID:  15708489     Owner:  NLM     Status:  MEDLINE    
The aim of this work was to study whether caffeine or theophylline chronically consumed during pregnancy affect inhibitory adenylyl cyclase pathway mediated by adenosine, in rat brain of both mothers and full-term fetuses. Immunoblotting analysis revealed a significant decrease in alphaGi(1,2) subunit level (27-29% in mothers, 15-18% in fetuses), associated with a significant increase in the mRNA level coding alphaGi(1) in both maternal and fetal rat brain (12-22%) after methylxanthine intake. No significant differences in alphaGi(3) level were detected in any case. On the other hand, forskolin- and forskolin plus guanosine-5'-O(3-thiotriphosphate) tetralithium salt-stimulated adenylyl cyclase activity was significantly decreased (30-36%) in maternal brain. Moreover, adenylyl cyclase inhibition elicited by N(6)-cyclohexyladenosine, specific adenosine A(1) receptor agonist, was also significantly decreased in caffeine- (40.5%) and theophylline- (55.0%) treated mothers, suggesting a desensitization of adenosine A(1) receptor/adenylyl cyclase pathway in maternal brain. However, no significant differences were detected in fetal brain between control and treated animals. Therefore, caffeine or theophylline chronic intake during pregnancy differently modulates inhibitory adenylyl cyclase pathway mediated by adenosine in maternal and fetal brain causing a loss of the system responsiveness only in maternal brain but down-regulating Gi(1) protein in both mother and fetus brain.
D León; J L Albasanz; M A Ruíz; M Martín
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience     Volume:  131     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  2005  
Date Detail:
Created Date:  2005-02-14     Completed Date:  2005-05-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  United States    
Other Details:
Languages:  eng     Pagination:  481-9     Citation Subset:  IM    
Area de Bioquímica, Facultad de Químicas, Centro Regional de Investigaciones Biomédicas, Universidad de Castilla-La Mancha, Avenida Camilo José Cela 10, 13071 Ciudad Real, Spain.
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MeSH Terms
Adenylate Cyclase / metabolism
Brain / drug effects*,  embryology,  physiology
Caffeine / administration & dosage*
Prenatal Exposure Delayed Effects*
Rats, Wistar
Receptor, Adenosine A1 / antagonists & inhibitors*,  physiology*
Theophylline / administration & dosage*
Reg. No./Substance:
0/Receptor, Adenosine A1; 58-08-2/Caffeine; 58-55-9/Theophylline; EC Cyclase

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