Document Detail


Chronic application of nonylphenol-induced apoptosis via suppression of bcl-2 transcription and up-regulation of active caspase-3 in mouse brain.
MedLine Citation:
PMID:  18514416     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonylphenol (NP) is an endocrine disruptor, which has been reported to have adverse effects on reproductive and immune systems. However, the influence of NP on the central nervous system (CNS) has not been extensively explored. The present study was performed to investigate the effects of chronic administration of NP on the apoptosis-related protein expression in mouse brain by in situ hybridization, RT-PCR and immunoblotting assays. The expression of bcl-2 mRNA was down-regulated by NP at the doses of 100 and 200mg/(kg day) (p<0.05), whereas the expression of bax mRNA was not affected in NP treated mice (p>0.05). Furthermore, as the main executor of apoptosis, the expression of active caspase-3 was up-regulated by 100 and 200mg/(kg day) NP (p<0.01), which is in accord with the results of terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) (p<0.05). These results suggest that chronic application of NP could sensitize the mice brain to apoptosis.
Authors:
Zhen Mao; Yuan-Lin Zheng; Yan-qiu Zhang; Bao-ping Han; Ling-tong Chen; Jing Li; Fei Li; Qun Shan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-08
Journal Detail:
Title:  Neuroscience letters     Volume:  439     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-10     Completed Date:  2008-10-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  147-52     Citation Subset:  IM    
Affiliation:
School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou 221008, Jiangsu Province, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / drug effects*
Brain / drug effects*,  metabolism
Caspase 3 / genetics,  metabolism*
Dose-Response Relationship, Drug
Drug Administration Schedule
Male
Mice
Phenols / administration & dosage*
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism*
RNA, Messenger / metabolism
Up-Regulation / drug effects*
Chemical
Reg. No./Substance:
0/Phenols; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 25154-52-3/nonylphenol; EC 3.4.22.-/Caspase 3

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