Document Detail


Chronic alcohol consumption is associated with changes in the distribution, immunophenotype, and the inflammatory cytokine secretion profile of circulating dendritic cells.
MedLine Citation:
PMID:  17386065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Alcoholism is frequently associated with altered immune responses, limited information being available on its effects on dendritic cells (DC). In the present study we analyze the effects of chronic alcoholism on circulating DC. METHODS: For the first time we studied the numerical distribution of DC in peripheral blood (PB), their immunophenotype, and their ex vivo pattern of spontaneous cytokine secretion, in chronic alcoholic patients without liver disease (AWLD group; n=17) and active ethanol (EtOH) intake, as well as in subjects with alcohol liver cirrhosis (ALC group; n=21). RESULTS: A significantly decreased HLADR expression and an increased reactivity for CD123 was observed on PB DC from AWLD patients; additionally, increased secretion of interleukin (IL) 1beta, IL6, IL12, and tumor necrosis factor-alpha (TNFalpha) by DC was also noted in this group. Conversely, patients with ALC and at least 1 year of alcohol withdrawal (ALCAW group) showed a decreased number of total circulating DC, whereas ALC patients with active EtOH intake (ALCET group) had an abnormally low production of IL1beta and TNFalpha by PB DC. CONCLUSION: Chronic alcoholism in the absence of liver disease is associated with an increased secretion of inflammatory cytokines by PB DC, whereas ALCAW and ALCET patients show decreased numbers of circulating DC and reduced secretion of these cytokines, respectively.
Authors:
Francisco Javier Laso; José Miguel Vaquero; Julia Almeida; Miguel Marcos; Alberto Orfao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-26
Journal Detail:
Title:  Alcoholism, clinical and experimental research     Volume:  31     ISSN:  0145-6008     ISO Abbreviation:  Alcohol. Clin. Exp. Res.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-17     Completed Date:  2007-05-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7707242     Medline TA:  Alcohol Clin Exp Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  846-54     Citation Subset:  IM    
Affiliation:
Unidad de Alcoholismo, Servicio de Medicina Interna II, Hospital Universitario de Salamanca, Salamanca, Spain. laso@usal.es
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MeSH Terms
Descriptor/Qualifier:
Adult
Alcoholism / immunology,  metabolism*
Antigens, CD / metabolism
Antigens, Differentiation, Myelomonocytic / metabolism
Cell Count
Cytokines / metabolism*
Dendritic Cells / classification,  immunology,  metabolism*
Humans
Inflammation / immunology,  metabolism*
Interleukin-3 Receptor alpha Subunit / metabolism
Liver Cirrhosis, Alcoholic / immunology,  pathology
Male
Phenotype
Receptors, IgG / metabolism
Substance Withdrawal Syndrome / metabolism
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, Differentiation, Myelomonocytic; 0/CD33 antigen; 0/Cytokines; 0/Interleukin-3 Receptor alpha Subunit; 0/Receptors, IgG

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