Document Detail

Chronic acid water feeding protects mice against lethal gut-derived sepsis due to Pseudomonas aeruginosa.
MedLine Citation:
PMID:  16570696     Owner:  NLM     Status:  MEDLINE    
Acidified feeding formulas have been proposed as a method of controlling gastrointestinal colonization and nosocomial infection in critically ill patients. We examined possible mechanisms by which chronic acid water feeding might protect the host against lethal gut derived sepsis by assessing its effect on both local intestinal epithelial barrier function to bacteria as well as on local and systemic heat shock protein expression. Heat shock protein expression measured by immunoblot demonstrated that HSP25 was increased in the stomach, aorta and kidney of mice chronically fed acid water (8 weeks) compared to tap water fed controls. HSP72 expression was also increased in the aorta of mice drinking acid water. The protein content of cecum and its barrier function were enhanced in mice ingesting acidified water. The direct effect of an acid environment on intestinal epithelial barrier function was tested in cultured human intestinal epithelial cells. An acidified environment protected against bacterial mediated disruption of the intestinal epithelial barrier. Finally, the protective effect of chronic acid water feeding on gut-derived sepsis due to P. aeruginosa was tested in mice. Chronic acid water feeding protected mice from the lethal gut derived sepsis due to P. aeruginosa.
Licheng Wu; Jonathan E Kohler; Olga Zaborina; Garg Akash; Mark W Musch; Eugene B Chang; John C Alverdy
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current issues in intestinal microbiology     Volume:  7     ISSN:  1466-531X     ISO Abbreviation:  Curr Issues Intest Microbiol     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-30     Completed Date:  2006-06-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100939156     Medline TA:  Curr Issues Intest Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  19-28     Citation Subset:  IM    
Department of Surgery, University of Chicago, Illinois, USA.
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MeSH Terms
Anti-Bacterial Agents / administration & dosage,  pharmacology
Aorta / chemistry
Caco-2 Cells
Cecum / chemistry
Disease Models, Animal
Epithelial Cells / microbiology
Heat-Shock Proteins / analysis,  biosynthesis
Hydrogen-Ion Concentration
Intestinal Mucosa / physiology
Kidney / chemistry
Mice, Inbred BALB C
Proteins / analysis
Pseudomonas Infections / prevention & control*
Pseudomonas aeruginosa*
Sepsis / prevention & control*
Stomach / chemistry
Water / administration & dosage*,  chemistry*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Heat-Shock Proteins; 0/Proteins; 7732-18-5/Water

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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