| Chronic alcohol exposure stimulates adipose tissue lipolysis in mice: role of reverse triglyceride transport in the pathogenesis of alcoholic steatosis. | |
| | |
MedLine Citation:
|
PMID: 22234172 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Alcohol consumption induces liver steatosis; therefore, this study investigated the possible role of adipose tissue dysfunction in the pathogenesis of alcoholic steatosis. Mice were pair-fed an alcohol or control liquid diet for 8 weeks to evaluate the alcohol effects on lipid metabolism at the adipose tissue-liver axis. Chronic alcohol exposure reduced adipose tissue mass and adipocyte size. Fatty acid release from adipose tissue explants was significantly increased in alcohol-fed mice in association with the activation of adipose triglyceride lipase and hormone-sensitive lipase. Alcohol exposure induced insulin intolerance and inactivated adipose protein phosphatase 1 in association with the up-regulation of phosphatase and tensin homolog (PTEN) and suppressor of cytokine signaling 3 (SOCS3). Alcohol exposure up-regulated fatty acid transport proteins and caused lipid accumulation in the liver. To define the mechanistic link between adipose triglyceride loss and hepatic triglyceride gain, mice were first administered heavy water for 5 weeks to label adipose triglycerides with deuterium, and then pair-fed alcohol or control diet for 2 weeks. Deposition of deuterium-labeled adipose triglycerides in the liver was analyzed using Fourier transform ion cyclotron mass spectrometry. Alcohol exposure increased more than a dozen deuterium-labeled triglyceride molecules in the liver by up to 6.3-fold. These data demonstrate for the first time that adipose triglycerides due to alcohol-induced hyperlipolysis are reverse transported and deposited in the liver. |
| | |
Authors:
|
Wei Zhong; Yantao Zhao; Yunan Tang; Xiaoli Wei; Xue Shi; Wenlong Sun; Xiuhua Sun; Xinmin Yin; Xinguo Sun; Seongho Kim; Craig J McClain; Xiang Zhang; Zhanxiang Zhou |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-01-09 |
Journal Detail:
|
Title: The American journal of pathology Volume: 180 ISSN: 1525-2191 ISO Abbreviation: Am. J. Pathol. Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-02-20 Completed Date: 2012-04-27 Revised Date: 2012-05-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370502 Medline TA: Am J Pathol Country: United States |
Other Details:
|
Languages: eng Pagination: 998-1007 Citation Subset: AIM; IM |
Copyright Information:
|
Copyright © 2012 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Nutrition, University of North Carolina at Greensboro, Greensboro, North Carolina 28081, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipose Tissue
/
drug effects* Animals Chronic Disease Deuterium Oxide / diagnostic use Down-Regulation Ethanol / administration & dosage, toxicity* Fatty Acids / metabolism Fatty Liver, Alcoholic / etiology* Homeostasis / drug effects Insulin Resistance / physiology Lipid Metabolism / drug effects Lipolysis / drug effects* Liver / metabolism Male Mice Mice, Inbred C57BL Organ Size PTEN Phosphohydrolase / metabolism Suppressor of Cytokine Signaling Proteins / metabolism Triglycerides / metabolism Up-Regulation |
| Grant Support | |
ID/Acronym/Agency:
|
1RC2AA019385/AA/NIAAA NIH HHS; P01AA017103/AA/NIAAA NIH HHS; P30AA019360/AA/NIAAA NIH HHS; R01 GM087735-03/GM/NIGMS NIH HHS; R01 GM087735-04/GM/NIGMS NIH HHS; R01AA015970/AA/NIAAA NIH HHS; R01AA018016/AA/NIAAA NIH HHS; R01AA018844/AA/NIAAA NIH HHS; R01AA018869/AA/NIAAA NIH HHS; R01DK7071765/DK/NIDDK NIH HHS; R01GM087735/GM/NIGMS NIH HHS; R37AA010762/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids; 0/Socs3 protein, mouse; 0/Suppressor of Cytokine Signaling Proteins; 0/Triglycerides; 64-17-5/Ethanol; 7789-20-0/Deuterium Oxide; EC 3.1.3.67/PTEN Phosphohydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Growth Defects and Impaired Cognitive-Behavioral Abilities in Mice Knockout for Eif4h, a Gene Locate...
Next Document: Cathepsin cleavage of sirtuin 1 in endothelial progenitor cells mediates stress-induced premature se...