Document Detail


Chromosome translocation induced by the insertion of the URA blaster into the major repeat sequence (MRS) in Candida albicans.
MedLine Citation:
PMID:  15197728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electrophoretic karyotype studies have shown that clinical isolates of Candida albicans have extensive chromosome length polymorphisms. Chromosome translocation is one of the causes of karyotypic variation. Chromosome translocation events have been shown to occur very frequently at or near the major repeat sequence (MRS) on chromosomes. The MRS consists of the repeated sequences RB2, RPS and HOK, and the repeated sequences are considered to be the template for recombination. To investigate which element of the MRS is important for chromosome translocation, we constructed three cassettes, each containing a URA blaster and sequences homologous to one of the repeats, for insertion into the MRS region on the chromosomes. The ura3 strain STN22u2, which shows a stable, standard karyotype, was transformed with each construct. Insertion events with each cassette occurred at almost all chromosomes. Insertion into the RB2 repeat, but not into the RPS repeat, was accompanied by chromosome translocation in some transformants: chromosome translocations between chromosomes R and 7 and chromosomes 1 and 7 were found, as well as deletions of 7A and 7C from chromosome 7. We conclude that the insertion at the RB2 region may initiate chromosome translocation in C. albicans.
Authors:
Shin-Ichi Iwaguchi; Mina Suzuki; Naomi Sakai; Yoshiyuki Nakagawa; Paul T Magee; Takahito Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  21     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-15     Completed Date:  2004-09-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  England    
Other Details:
Languages:  eng     Pagination:  619-34     Citation Subset:  IM    
Copyright Information:
Copyright 2004 John Wiley & Sons, Ltd.
Affiliation:
Department of Biological Science, Nara Women's University, Nara 630-8506, Japan. iwaguchi@cc.nara-wu.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / genetics*
Chromosomes
Genome, Fungal*
Phenotype
Terminal Repeat Sequences / genetics*
Translocation, Genetic*
Grant Support
ID/Acronym/Agency:
AI16567/AI/NIAID NIH HHS; AI46351/AI/NIAID NIH HHS

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